1887

Abstract

Two strains of Gram-negative, aerobic, non-pigmented, non-motile, rod-shaped, methane-oxidizing bacteria were isolated from an acidic forest cambisol near Marburg, Germany, and were designated as strains BL2 and A1. These bacteria were morphologically and phenotypically similar to K. The cells possess a highly specific bipolar appearance. They lack the intracytoplasmic membranes common to all methane-oxidizing bacteria except , but contain a vesicular membrane system connected to the cytoplasmic membrane. A soluble methane monooxygenase was present, but no particulate methane monooxygenase could be detected. These bacteria utilize the serine pathway for carbon assimilation. Strains BL2 and A1 are moderately acidophilic, mesophilic organisms capable of growth at pH values between 4·5 and 7 (with an optimum at pH 5·5) and at temperatures between 4 and 30 °C. Compared with K, these strains have greater tolerance of cold temperatures, dissolved salts and methanol. On the basis of 16S rRNA gene sequence identity, of species with validly published names, strain BL2 is most closely related to K (97·3 % identity), subsp. ATCC 9039 (97·1 %) and B2 (96·2 %). The DNA G+C content is 60 mol% and the major phospholipid fatty acid is 18 : 17. Strain BL2 showed only 21–22 % DNA–DNA hybridization with K. The data therefore suggest that strains BL2 and A1 represent a novel species of ; the name sp. nov. is proposed, with strain BL2 (=DSM 15510=NCIMB 13906) as the type strain.

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2003-09-01
2019-08-21
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