1887

Abstract

A novel anaerobic, heterotrophic thermophile was isolated from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Izu-Bonin Arc, Japan. The cells were bent, flexible rods, with a single polar flagellum. Growth was observed between 40 and 70 °C (optimum temperature: 60–65 °C; doubling time, 40 min) and between pH 5·0 and 7·5 (optimum pH 6·5). The isolate was a strictly anaerobic heterotroph capable of using complex organic compounds (yeast extract, tryptone, peptone, casein and Casamino acids), ethanol and various organic acids as energy and carbon sources. Hydrogen could serve as a supplementary energy source. Elemental sulfur (S), nitrate or arsenate was required for growth as an electron acceptor. The G+C content of the genomic DNA was 38·6 mol%. Phylogenetic analysis based on 16S rDNA sequences indicated that isolate SSM1 is closely related to BMA (98·1 %). However, the novel isolate could be clearly differentiated from BMA on the basis of its physiological and genetic properties. The name sp. nov. (type strain SSM1=JCM 11476=DSM 14783) is proposed.

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2003-05-01
2020-01-18
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Electron micrograph of negatively stained cells of strain SSM1 in the mid-exponential (A) and stationary (B) phases of growth. Bars, 1 µm.

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Effects of temperature (C), pH (D) and sea salt concentration (E) on the growth of strain SSM1 . Growth curves at different temperatures were determined in the standard medium at pH 6.5. The effect of pH on growth was determined in the standard medium containing various buffers at 62 °C over a range of pH values. The pH values were adjusted to various levels at room temperature. The effect of sea salt concentration on growth was determined in varying dilutions of 4x MJ synthetic sea water. [PDF](15 KB)

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