A Gram-positive, moderately halophilic and endospore-forming bacterium, designated strain 18OMT, was isolated from salted animal hides. The cells were rods and produced ellipsoidal endospores at a terminal position. Strain 18OMT was motile, strictly aerobic and grew at 0.5–25 % (w/v) NaCl [optimal growth at 10 % (w/v) NaCl], at between pH 5.0 and 9.0 (optimal growth at pH 7.5) and at temperatures between 15 and 45 °C (optimal growth at 37 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain 18OMT was closely related to species of the genus Thalassobacillus within the phylum Firmicutes. The closest phylogenetic similarity was with Thalassobacillus devorans G-19.1T (98.4 %), Thalassobacillus cyri HS286T (97.9 %) and Thalassobacillus hwangdonensis AD-1T (97.4 %). The major cellular fatty acids were anteiso-C15 : 0 (57.9 %), anteiso-C17 : 0 (14.0 %), iso-C15 : 0 (10.8 %) and iso-C16 : 0 (8.1 %). The respiratory isoprenoid quinones were MK-7 (98.5 %) and MK-6 (1.5 %). The DNA G+C content was 42.9 mol%. These features confirmed the placement of strain 18OMT within the genus Thalassobacillus. The DNA–DNA hybridization values between strain 18OMT and T. devorans G-19.1T, T. cyri HS286T and T. hwangdonensis AD-1T were 49 %, 9 % and 15 %, respectively, showing unequivocally that strain 18OMT constituted a novel genospecies. On the basis of phylogenetic analysis and phenotypic, genotypic and chemotaxonomic characteristics, strain 18OMT is considered to represent a novel species of the genus Thalassobacillus, for which the name Thalassobacillus pellis sp. nov. is proposed. The type strain is 18OMT ( = CECT 7566T = DSM 22784T = JCM 16412T).
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Funding
This study was supported by the:
Spanish Ministerio de Educación y Ciencia
(Award BIO2009-10138)
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