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Volume 53, Issue 5

Isolation of thermophilic strains with methanol and sulfite from solfataric mud pools, and characterization of sp. nov. Free

Abstract

Four strains of thermophilic, endospore-forming, sulfate-reducing bacteria were enriched and isolated from hot solfataric fields in the Krafla area of north-east Iceland, using methanol and sulfite as substrates. Morphologically, these strains resembled thermophilic species. The strains grew with alcohols, including methanol, with glucose and fructose as electron donors, and with sulfate, sulfite or thiosulfate as electron acceptors. For all four strains, the optimum temperature and pH for growth were 60 °C and pH 7·3, respectively; no added NaCl was required. Phylogenetic analysis based on partial 16S rRNA gene sequence comparisons showed high levels of similarity of the novel strains (>92 %) with and . However, DNA–DNA hybridization studies with revealed that the four strains belonged to one novel species. A representative of this group of isolates, strain V21, is proposed as the type strain of a novel species of the spore-forming, sulfate-reducing genus , namely (type strain V21=DSM 14956=CIP 107984).

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2003-09-01
2022-01-22
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Isolation of thermophilic Desulfotomaculum strains with methanol and sulfite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum sp. nov.
Int J Syst Evol Microbiol 53, 1223 (2003); https://doi.org/10.1099/ijs.0.02476-0
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