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Abstract

An actinobacterial strain, designated G4, isolated from a coal mine was subjected to polyphasic taxonomic characterization. Cells were Gram-stain-positive, yellow-pigmented, non-motile and non-spore-forming cocci. This organism possessed a type B peptidoglycan with diaminobutyric acid as diagnostic diamino acid. The major respiratory quinones were MK-9, MK-10 and MK-11. The major fatty acids were anteiso-C (41.6  %) and anteiso-C (32.8 %). The predominant cellular polar lipids were diphosphatidylglycerol and phosphatidylglycerol. Cell wall sugars comprised galactose, glucose, ribose and rhamnose. 16S rRNA gene sequence analysis of strain G4 showed high similarity with (98.9 %), (97.8 %), (97.3 %) and (97.0 %). Sequence similarity with the type strains of the other species of the genus was less than 97.0 %. The DNA–DNA relatedness of strain G4 with the type strains of , and was less than 70 %. On the basis of the physiological, biochemical and chemotaxonomic characteristics, strain G4 should be classified as the type strain of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is G4 ( = MTCC 10213  = DSM 22965).

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2011-06-01
2020-01-23
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vol. , part 6, pp. 1253 - 1258

Total polar lipid profile of strain G4 . DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; GL, glycolipids; UL, unknown lipids.

Utilization of various substrates as determined using Biolog GP2 microplates

Strains: 1, G4 ; 2, MTCC 6457 ; 3, MTCC 6410 ; 4, MTCC 6413 ; 5, MTCC 10319 . +, Positive; −, negative. In Biolog GP2 tests, all strains are positive for oxidation of l-arabinose, d-fructose, d-gluconic acid, α- d-glucose, d-mannose, d-psicose, d-ribose, β-hydroxybutyric acid, -hydroxyphenylacetic acid, glycerol and adenosine, and negative for α-cyclodextrin, β-cyclodextrin, glycogen, inulin, mannan, -acetyl- d-glucosamine, -acetyl-β- d-mannosamine, amygdalin, d-arabitol, arbutin, cellobiose, l-fucose, gentiobiose, -inositol, lactose, lactulose, maltotriose, melezitose, melibiose, β-methyl d-galactoside, 3-methyl d-glucose, β-methyl d-glucoside, α-methyl d-mannoside, stachyose, trehalose, xylitol, acetic acid, α-hydroxybutyric acid, lactamide, d-lactic acid methyl ester, d-malic acid, succinamic acid, succinic acid, -acetyl- l-glutamic acid, l-alaninamide, d-alanine, l-alanine, l-alanyl glycine, l-asparagine, glycyl l-glutamic acid, l-pyroglutamic acid, 2,3-butanediol, α- d-glucose 1-phosphate, d-glucose 6-phosphate (data from the present study).

Comparative fatty acid profiles of strain G4 and related type strains

Strains: 1, G4 ; 2, MTCC 6457 ; 3, MTCC 6410 ; 4, MTCC 6413 ; 5, MTCC 10319 . All data from this study. Values are percentages of total fatty acids; nd, not detected.



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