1887

Abstract

A Gram-reaction-negative, strictly aerobic, rod-shaped, non-motile strain, designated 2-1-2, was isolated from perchloroethylene/trichloroethene-contaminated soil in Suwon, South Korea. A polyphasic approach was used to study the taxonomic position of strain 2-1-2. Strain 2-1-2 showed highest 16S rRNA gene sequence similarities to TR6-04 (97.9 %) and ATCC 33299 (97.1 %); sequence similarities to other species were less than 93.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 2-1-2 belonged to the clade formed by members of the genus in the family . The G+C content of the genomic DNA was 36.6 mol%. Strain 2-1-2 showed the typical chemotaxonomic features of the genus , with the presence of a ceramide phosphorylethanolamine (CerPE-2) as the major ceramide, menaquinone 7 (MK-7) as the predominant respiratory quinone and iso-C, iso-C 3-OH and summed feature 3 (comprising iso-C 2-OH and/or Cω7) as the major fatty acids. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, and DNA–DNA relatedness, strain 2-1-2 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is 2-1-2 ( = KEMC 2241-005 = JCM 16704). Emended descriptions of and are also proposed.

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2012-11-01
2019-12-15
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