1887

Abstract

Seven closely related xylanolytic, thermophilic bacilli were isolated from mud and water samples from the Gonen and Diyadin hot springs, respectively located in the Turkish provinces of Balikesir and Agri. On the basis of morphology and biochemical characteristics, one of the isolates, designated strain G2, was studied further. Strain G2 is a xylanolytic, sporulating, Gram-positive, rod-shaped bacterium. The isolate is a thermophilic (optimum temperature for growth, 55–60 °C), facultative anaerobe that grows on a wide range of carbon sources, including glucose, starch, xylose and mannitol. It expressed a high level of xylose isomerase activity on xylose and also on glucose. 16S rRNA gene sequence analysis showed that this isolate resembled DSM 2641 (>97 % similarity), but 16S–23S rDNA internally transcribed spacer polymorphism PCR showed variation between DSM 2641 and isolate G2. However, it is also known that analysis of 16S rRNA gene sequences may be insufficient to distinguish between some species. In DNA–DNA hybridization, thermophilic isolate G2 showed relatedness of 53·4 % to and about 45·0 % to , indicating that it is distinct at the species level. On the basis of the evidence presented, it is proposed that strain G2 (=NCIMB 13933=NCCB 100040) be designated as the type strain of sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02473-0
2003-09-01
2019-10-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/5/ijs531315.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02473-0&mimeType=html&fmt=ahah

References

  1. Ahmad, S., Scopes, R. K., Rees, G. N. & Patel, B. K. C. ( 2000; ). Saccharococcus caldoxylolyticus sp. nov., an obligately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 50, 517–523.[CrossRef]
    [Google Scholar]
  2. Ash, C., Farrow, J. A. E., Wallbanks, S. & Collins, M. D. ( 1991; ). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13, 202–206.
    [Google Scholar]
  3. Beffa, T., Blanc, M., Lyon, P. F., Vogt, G., Marchiani, M., Fischer, J. L. & Aragno, M. ( 1996; ). Isolation of Thermus strains from hot composts (60 to 80 °C). Appl Environ Microbiol 62, 1723–1727.
    [Google Scholar]
  4. Belduz, A. O., Lee, E. J. & Harman, J. G. ( 1993; ). Mutagenesis of the cyclic AMP receptor protein of Escherichia coli: targeting positions 72 and 82 of the cyclic nucleotide binding pocket. Nucleic Acids Res 21, 1827–1835.[CrossRef]
    [Google Scholar]
  5. Bradford, M. M. ( 1976; ). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.[CrossRef]
    [Google Scholar]
  6. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  7. Cowan, S. T. & Steel, K. J. ( 1974; ). Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge: Cambridge University Press.
  8. Daffonchio, D., Borin, S., Frova, G., Manachini, P. L. & Sorlini, C. ( 1998; ). PCR fingerprinting of whole genomes: the spacers between the 16S and 23S rRNA genes and of intergenic tRNA gene regions reveal a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniformis. Int J Syst Bacteriol 48, 107–116.[CrossRef]
    [Google Scholar]
  9. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  10. Escara, J. F. & Hutton, J. R. ( 1980; ). Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19, 1315–1327.[CrossRef]
    [Google Scholar]
  11. Felsenstein, J. ( 1989; ). phylip: phylogeny inference package. (version 3.2). Cladistics 5, 164–166.
    [Google Scholar]
  12. Guagliardi, A., Martino, M., Iaccarino, I., de Rosa, M., Rossi, M. & Bartolucci, S. ( 1996; ). Purification and characterization of the alcohol dehydrogenase from a novel strain of Bacillus stearothermophilus growing at 70 °C. Int J Biochem Cell Biol 28, 239–246.[CrossRef]
    [Google Scholar]
  13. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  14. Johnson, J. L. ( 1985; ). Determination of DNA base composition. Methods Microbiol 18, 1–29.
    [Google Scholar]
  15. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  16. Lee, C. Y., Bagdasarian, M., Meng, M. H. & Zeikus, J. G. ( 1990; ). Catalytic mechanism of xylose (glucose) isomerase from Clostridium thermosulfurogenes. Characterization of the structural gene and function of active site histidine. J Biol Chem 265, 19082–19090.
    [Google Scholar]
  17. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  18. Miquel, P. ( 1888; ). Monographie d'un bacille vivant au-dela de 70 °C. Ann Micrographic 1, 3.
    [Google Scholar]
  19. Pikuta, E., Lysenko, A., Chuvilskaya, N., Mendrock, U., Hippe, H., Suzina, N., Nikitin, D., Osipov, G. & Laurinavichius, K. ( 2000; ). Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov. Int J Syst Evol Microbiol 50, 2109–2117.[CrossRef]
    [Google Scholar]
  20. Riffard, S., Lo Presti, F., Normand, P., Forey, F., Reyrolle, M., Etienne, J. & Vandenesch, F. ( 1998; ). Species identification of Legionella via intergenic 16S–23S ribosomal spacer PCR analysis. Int J Syst Bacteriol 48, 723–730.[CrossRef]
    [Google Scholar]
  21. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  22. Vandamme, P., Pot, B., Gillis, M., De Vos, P., Kersters, K. & Swings, J. ( 1996; ). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407–438.
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02473-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02473-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error