1887

Abstract

A Gram-negative, non-motile, non-spore-forming, halophilic rod, designated JPCCMB0017, was isolated from a marine sediment of the coastal area of Okinawa, Japan. The isolate formed orange–red colonies on marine agar. Bacteriochlorophyll α was absent and sphingoglycolipid 1 and other carotenoids, including astaxanthin, adonixanthin and zeaxanthin, were present. Ubiquinone-10 (Q-10) was the main respiratory quinone and Cω7 was the major cellular fatty acid. The G+C content of DNA was 59.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus in the family . Strain JPCCMB0017 exhibited 96.8 % 16S rRNA gene sequence similarity with H32. Unlike other members of the genus , strain JPCCMB0017 reduced nitrate. On the basis of genotypic and phenotypic data, a novel species is proposed to accommodate this isolate, with the name sp. nov. The type strain is JPCCMB0017 ( = NITE-AP48 = ATCC BAA-2084 = NBRC 107699).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.024729-0
2011-12-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/12/2956.html?itemId=/content/journal/ijsem/10.1099/ijs.0.024729-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Andrewes A. G. , Phaff H. J. , Starr M. P. . ( 1976; ). Carotenoids of Phaffia rhodozyma, a red-pigmented fermenting yeast. . Phytochemistry 15:, 1003–1007. [CrossRef]
    [Google Scholar]
  3. Asker D. , Beppu T. , Ueda K. . ( 2007; ). Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan. . FEMS Microbiol Lett 273:, 140–148. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bar E. , Rise M. , Vishkautsan M. , Arad S. . ( 1995; ). Pigment and structural changes in Chlorella zofingiensis upon light and nitrogen stress. . J Plant Physiol 146:, 527–534.[CrossRef]
    [Google Scholar]
  5. Fan Z.-Y. , Xiao Y.-P. , Hui W. , Tian G.-R. , Lee J.-S. , Lee K. C. , Quan Z.-X. . ( 2011; ). Altererythrobacter dongtanensis sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 61:, 2035–2039. [CrossRef] [PubMed]
    [Google Scholar]
  6. Hiraishi A. , Imhoff J. F. . ( 2005; ). Genus VII. Porphyrobacter Fuerst, Hawkins, Holms, Sly, Moore and Stackebrandt 1993, 132VP . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2C, pp. 275–279. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  7. Iizuka H. , Nishimura Y. . ( 1969; ). Microbiological studies on petroleum and natural gas. X. Carotenoid pigments of hydrocarbon-utilizing bacteria. . J Gen Appl Microbiol 15:, 127–134. [CrossRef]
    [Google Scholar]
  8. Katayama-Fujimura Y. , Komatsu Y. , Kuraishi H. , Kaneko T. . ( 1984; ). Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. . Agric Biol Chem 48:, 3169–3172. [CrossRef]
    [Google Scholar]
  9. Kawahara K. , Seydel U. , Matsuura M. , Danbara H. , Rietschel E. T. , Zähringer U. . ( 1991; ). Chemical structure of glycosphingolipids isolated from Sphingomonas paucimobilis . . FEBS Lett 292:, 107–110. [CrossRef]
    [Google Scholar]
  10. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kumar N. R. , Nair S. , Langer S. , Busse H.-J. , Kämpfer P. . ( 2008; ). Altererythrobacter indicus sp. nov., isolated from wild rice (Porteresia coarctata Tateoka). . Int J Syst Evol Microbiol 58:, 839–844. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kwon K. K. , Woo J.-H. , Yang S.-H. , Kang J.-H. , Kang S. G. , Kim S.-J. , Sato T. , Kato C. . ( 2007; ). Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov.. Int J Syst Evol Microbiol 57:, 2207–2211. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lai Q. , Yuan J. , Shao Z. . ( 2009; ). Altererythrobacter marinus sp. nov., isolated from deep seawater. . Int J Syst Evol Microbiol 59:, 2973–2976. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lorenz R. T. , Cysewski G. R. . ( 2000; ). Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. . Trends Biotechnol 18:, 160–167. [CrossRef] [PubMed]
    [Google Scholar]
  15. Nishijima M. , Araki-Sakai M. , Sano H. . ( 1997; ). Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. . J Microbiol Methods 28:, 113–122. [CrossRef]
    [Google Scholar]
  16. Pane L. , Radin L. , Franconi G. , Carli A. . ( 1996; ). The carotenoid pigments of a marine Bacillus firmus strain. . Boll Soc Ital Biol Sper 72:, 303–308.[PubMed]
    [Google Scholar]
  17. Park S. C. , Baik K. S. , Choe H. N. , Lim C. H. , Kim H. J. , Ka J.-O. , Seong C. N. . ( 2011; ). Altererythrobacter namhicola sp. nov. and Altererythrobacter aestuarii sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61:, 709–715. [CrossRef] [PubMed]
    [Google Scholar]
  18. Renstrom B. , Borch G. , Skulberg O. M. , Liaaenjensen S. . ( 1981; ). Natural occurrence of enantiomeric and meso-astaxanthin. 3. Optical purity of (3S,3′S)-astaxanthin from Haematococcus pluvialis . . Phytochemistry 20:, 2561–2564.[CrossRef]
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Sambrook J. , Fritsch E. F. , Maniatis T. . ( 1989; ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  21. Seo S. H. , Lee S. D. . ( 2010; ). Altererythrobacter marensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 60:, 307–311. [CrossRef] [PubMed]
    [Google Scholar]
  22. Shiba T. , Simidu U. . ( 1982; ). Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll α. . Int J Syst Bacteriol 32:, 211–217. [CrossRef]
    [Google Scholar]
  23. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tsubokura A. , Yoneda H. , Mizuta H. . ( 1999; ). Paracoccus carotinifaciens sp. nov., a new aerobic Gram-negative astaxanthin-producing bacterium. . Int J Syst Bacteriol 49:, 277–282. [CrossRef] [PubMed]
    [Google Scholar]
  25. Xu X.-W. , Wu Y.-H. , Wang C.-S. , Wang X.-G. , Oren A. , Wu M. . ( 2009; ). Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae . . Int J Syst Evol Microbiol 59:, 2247–2253. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yabuuchi E. , Yano I. , Oyaizu H. , Hashimoto Y. , Ezaki T. , Yamamoto H. . ( 1990; ). Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas . . Microbiol Immunol 34:, 99–119.[PubMed] [CrossRef]
    [Google Scholar]
  27. Yabuuchi E. , Kosako Y. , Naka T. , Suzuki S. , Yano I. . ( 1999; ). Proposal of Sphingomonas suberifaciens (van Bruggen, Jochimsen and Brown 1990) comb. nov., sphingomonas natatoria (Sly 1985) comb. nov., Sphingomonas ursincola (Yurkov et al. 1997) comb. nov., and emendation of the genus Sphingomonas . . Microbiol Immunol 43:, 339–349.[PubMed] [CrossRef]
    [Google Scholar]
  28. Yokota A. , Akagawa-Matsushita M. , Hiraishi A. , Katayama Y. , Urakami T. , Yamasato K. . ( 1992; ). Distributions of quinone systems in microorganisms: Gram-negative eubacteria. . Bull Jpn Fed Cult Coll 8:, 136–171.
    [Google Scholar]
  29. Yoon J.-H. , Kang K. H. , Yeo S.-H. , Oh T.-K. . ( 2005; ). Erythrobacter luteolus sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 55:, 1167–1170. [CrossRef] [PubMed]
    [Google Scholar]
  30. Yurkov V. , Stackebrandt E. , Holmes A. , Fuerst J. A. , Hugenholtz P. , Golecki J. , Gad’on N. , Gorlenko V. M. , Kompantseva E. I. , Drews G. . ( 1994; ). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44:, 427–434. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.024729-0
Loading
/content/journal/ijsem/10.1099/ijs.0.024729-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2956 - 2961

Ultrathin section of cells of strain JPCCMB0017 . Phenotypic characteristics of sp. nov. Carotenoid contents of sp. nov. and reference strains.

[ Combined PDF] 74 KB

 

 



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error