1887

Abstract

A strictly anaerobic, slightly halophilic and moderately thermophilic, sporulating rod designated strain DVird3 was isolated from deep-sea hydrothermal vent samples collected at a depth of approximately 800 m on the Atlantic Ocean Ridge. Strain DVird3 possessed a few laterally inserted flagella, had a DNA G+C content of 33·1 mol% and grew optimally at pH 6·6 and at 45 °C. Growth was observed at temperatures between 20 and 58 °C and at pH values between 5·8 and 8·2. The optimum NaCl concentration for growth was 3 % sea salt (30 g l); no growth was observed in the presence of 15 or 60 g sea salt l. Strain DVird3 is heterotrophic and utilizes some sugars and various single amino acids. Acetate was the main fatty acid detected from carbohydrate fermentation, together with H and CO. Gelatin was used as an energy source. It performed the Stickland reaction. Phylogenetically, strain DVird3 branched with members of cluster XI of the order , with as its closest relative (similarity of 94·6 %). On the basis of its phenotypic, genotypic and phylogenetic characteristics, strain DVird3 (=DSM 15212 =CIP 107654) is proposed as the type strain of a novel species of the genus , sp. nov.

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2003-07-01
2019-10-16
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References

  1. Alain, K., Querellou, J., Lesongeur, F., Pignet, P., Crassous, P., Raguénès, G., Cueff, V. & Cambon-Bonavita, M.-A. ( 2002a; ). Caminibacter hydrogeniphilus gen. nov., sp. nov., a novel thermophilic hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52, 1317–1323.[CrossRef]
    [Google Scholar]
  2. Alain, K., Pignet, P. Zbinden, M. & 8 other authors ( 2002b; ). Caminicella sporogenes gen. nov., sp. nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52, 1621–1628.[CrossRef]
    [Google Scholar]
  3. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  4. Andrews, K. T. & Patel, B. K. C. ( 1996; ). Fervidobacterium gondwanense sp. nov., a new thermophilic anaerobic bacterium isolated from nonvolcanically heated geothermal waters of the Great Artesian Basin of Australia. Int J Syst Bacteriol 46, 265–269.[CrossRef]
    [Google Scholar]
  5. Balch, W. E., Fox, G. E., Magrum, R. J. & Wolfe, R. S. ( 1979; ). Methanogens: reevaluation of a unique biological group. Microbiol Rev 43, 260–296.
    [Google Scholar]
  6. Benson, D. A., Boguski, M. S., Lipman, D. J., Ostell, J., Ouellette, B. F. F., Rapp, B. A. & Wheeler, D. L. ( 1999; ). GenBank. Nucleic Acids Res 27, 12–17.[CrossRef]
    [Google Scholar]
  7. Campbell, B. J., Jeanthon, C., Kotska, J. E., Luther, G. W., III & Cary, S. C. ( 2001; ). Growth and phylogenetic properties of novel bacteria belonging to the epsilon subdivision of the Proteobacteria enriched from Alvinella pompejana and deep-sea hydrothermal vents. Appl Environ Microbiol 67, 4566–4572.[CrossRef]
    [Google Scholar]
  8. Cato, E. & Stackebrandt, E. ( 1989; ). Taxonomy and phylogeny. In Clostridia, pp. 1–26. Edited by N. P. Minton & D. J. Clarke. New York: Plenum.
  9. Cato, E. P., George, W. L. & Finegold, S. M. ( 1986; ). Genus Clostridium. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1141–1200. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  10. Cayol, J.-L., Ollivier, B. Lawson, A. S. A. & 7 other authors ( 1994; ). Haloincola saccharolytica subsp. senegalensis subsp. nov., isolated from the sediments of a hypersaline lake, and emended description of Haloincola saccharolytica. Int J Syst Bacteriol 44, 805–811.[CrossRef]
    [Google Scholar]
  11. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  12. Corre, E., Reysenbach, A. L. & Prieur, D. ( 2001; ). ε-Proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. FEMS Microbiol Lett 205, 329–335.
    [Google Scholar]
  13. Fardeau, M.-L., Cayol, J.-L., Magot, M. & Ollivier, B. ( 1993; ). H2 oxidation in the presence of thiosulfate, by a Thermoanaerobacter strain isolated from an oil-producing well. FEMS Microbiol Lett 113, 327–332.[CrossRef]
    [Google Scholar]
  14. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  15. Felsenstein, J. ( 1993; ). phylip: phylogenetic inference package, version 3.5.1. Department of Genetics, University of Washington, Seattle, USA.
  16. Fendrich, C., Hippe, H. & Gottschalk, G. ( 1990; ). Clostridium halophilium sp. nov. and C. litorale sp. nov., an obligate halophilic and a marine species degrading betaine in the Stickland reaction. Arch Microbiol 154, 127–132.[CrossRef]
    [Google Scholar]
  17. Götz, D., Banta, A., Beveridge, T. J., Rushdi, A. I., Simoneit, B. R. T. & Reysenbach, A.-L. ( 2002; ). Persephonella marina gen. nov., sp. nov. and Persephonella guaymasensis sp. nov., two novel, thermophilic, hydrogen-oxidizing microaerophiles from deep-sea hydrothermal vents. Int J Syst Evol Microbiol 52, 1349–1359.[CrossRef]
    [Google Scholar]
  18. Haddad, A., Camacho, F., Durand, P. & Cary, S. C. ( 1995; ). Phylogenetic characterization of the epibiotic bacteria associated with the hydrothermal vent polychaete Alvinella pompejana. Appl Environ Microbiol 61, 1679–1687.
    [Google Scholar]
  19. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  20. Hernández-Eugenio, G., Fardeau, M.-L., Cayol, J.-L., Patel, B. K. C., Thomas, P., Macarie, H., Garcia, J.-L. & Ollivier, B. ( 2002; ). Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. Int J Syst Evol Microbiol 52, 1461–1468.[CrossRef]
    [Google Scholar]
  21. Hippe, H., Andreesen, J. R. & Gottschalk, G. ( 1992; ). The genus Clostridium – nonmedical. In The Prokaryotes, vol. II, pp. 1800–1866. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  22. Hungate, R. E. ( 1969; ). A roll-tube method for the cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  23. Jeanthon, C. ( 2000; ). Molecular ecology of hydrothermal vent microbial communities. Antonie van Leeuwenhoek 77, 117–133.[CrossRef]
    [Google Scholar]
  24. Jeanthon, C., L'Haridon, S., Reysenbach, A. L., Vernet, M., Messner, P., Sleytr, U. W. & Prieur, D. ( 1998; ). Methanococcus infernus sp. nov., a novel hyperthermophilic lithotrophic methanogen isolated from a deep-sea hydrothermal vent. Int J Syst Bacteriol 48, 913–919.[CrossRef]
    [Google Scholar]
  25. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 211–232. Edited by H. N. Munro. New York: Academic Press.
  26. Lawson, P. A., Perez, P. L., Hutson, R. A., Hippe, H. & Collins, M. D. ( 1993; ). Towards a phylogeny of the clostridia based on 16S rRNA sequences. FEMS Microbiol Lett 113, 87–92.[CrossRef]
    [Google Scholar]
  27. L'Haridon, S., Cilia, V., Messner, P., Raguénès, G., Gambacorta, A., Sleytr, U. W., Prieur, D. & Jeanthon, C. ( 1998; ). Desulfurobacterium thermolithotrophicum gen. nov., sp. nov., a novel autotrophic, sulfur-reducing bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Bacteriol 48, 701–711.[CrossRef]
    [Google Scholar]
  28. Love, C. A., Patel, B. K. C., Nichols, P. D. & Stackebrandt, E. ( 1993; ). Desulfotomaculum australicum sp. nov., a thermophilic sulfate-reducing bacterium isolated from the Great Artesian Basin of Australia. Syst Appl Microbiol 16, 244–251.[CrossRef]
    [Google Scholar]
  29. Maidak, B. L., Cole, J. R. Lilburn, T. G. & 7 other authors ( 2001; ). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef]
    [Google Scholar]
  30. Moore, S., Spackman, D. H. & Stein, W. H. ( 1958; ). Chromatography of amino acids on sulfonated polystyrene resins: an improved system. Anal Chem 30, 1185–1190.[CrossRef]
    [Google Scholar]
  31. Rainey, F. A. & Stackebrandt, E. ( 1993; ). 16S rRNA analysis reveals phylogenetic diversity among the polysaccharolytic clostridia. FEMS Microbiol Lett 113, 125–128.[CrossRef]
    [Google Scholar]
  32. Rainey, F. A., Ward, N. L., Morgan, H. W., Toalster, R. & Stackebrandt, E. ( 1993; ). Phylogenetic analysis of anaerobic thermophilic bacteria: aid for their reclassification. J Bacteriol 175, 4772–4779.
    [Google Scholar]
  33. Redburn, A. C. & Patel, B. K. C. ( 1993; ). Phylogenetic analysis of Desulfotomaculum thermobenzoicum using polymerase chain reaction-amplified 16S rRNA-specific DNA. FEMS Microbiol Lett 113, 81–86.[CrossRef]
    [Google Scholar]
  34. Reysenbach, A. L., Banta, A., Boone, D. R., Cary, S. C. & Luther, G. W. ( 2000a; ). Microbial essentials at hydrothermal vents. Nature 404, 835–836.[CrossRef]
    [Google Scholar]
  35. Reysenbach, A. L., Longnecker, K. & Kirshtein, J. ( 2000b; ). Novel bacterial and archaeal lineages from an in situ growth chamber deployed at the Mid-Atlantic Ridge hydrothermal vent. Appl Environ Microbiol 66, 3798–3806.[CrossRef]
    [Google Scholar]
  36. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 405–425.
    [Google Scholar]
  37. Stackebrandt, E. & Rainey, F. A. ( 1997; ). Phylogenetic relationships. In The Clostridia: Molecular Biology and Pathogenesis, pp. 3–19. Edited by J. I. Rood, B. A. McClane, J. G. Songer & R. W. Titball. New York: Academic Press.
  38. Wery, N., Moricet, J.-M., Cueff, V., Jean, J., Pignet, P., Lesongeur, F., Cambon-Bonavita, M.-A. & Barbier, G. ( 2001; ). Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 1789–1796.[CrossRef]
    [Google Scholar]
  39. Winker, S. & Woese, C. R. ( 1991; ). A definition of the domain Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. Syst Appl Microbiol 13, 161–165.[CrossRef]
    [Google Scholar]
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