@article{mbs:/content/journal/ijsem/10.1099/ijs.0.024661-0, author = "Chimetto, Luciane A. and Cleenwerck, Ilse and Brocchi, Marcelo and Willems, Anne and De Vos, Paul and Thompson, Fabiano L.", title = "Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2011", volume = "61", number = "5", pages = "1170-1175", doi = "https://doi.org/10.1099/ijs.0.024661-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.024661-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-negative, aerobic bacterium, designated strain R-40503T, was isolated from mucus of the reef-builder coral Mussismilia hispida, located in the São Sebastião Channel, São Paulo, Brazil. Phylogenetic analyses revealed that strain R-40503T belongs to the genus Marinomonas. The 16S rRNA gene sequence similarity of R-40503T was above 97 % with the type strains of Marinomonas vaga, M. basaltis, M. communis and M. pontica, and below 97 % with type strains of the other Marinomonas species. Strain R-40503T showed less than 35 % DNA–DNA hybridization (DDH) with the type strains of the phylogenetically closest Marinomonas species, demonstrating that it should be classified into a novel species. Amplified fragment length polymorphism (AFLP), chemotaxonomic and phenotypic analyses provided further evidence for the proposal of a novel species. Concurrently, a close genomic relationship between M. basaltis and M. communis was observed. The type strains of these two species showed 78 % DDH and 63 % AFLP pattern similarity. Their phenotypic features were very similar, and their DNA G+C contents were identical (46.3 mol%). Collectively, these data demonstrate unambiguously that Marinomonas basaltis is a later heterotypic synonym of Marinomonas communis. Several phenotypic features can be used to discriminate between Marinomonas species. The novel strain R-40503T is clearly distinguishable from its neighbours. For instance, it shows oxidase and urease activity, utilizes l-asparagine and has the fatty acid C12 : 1 3-OH but lacks C10 : 0 and C12 : 0. The name Marinomonas brasilensis sp. nov. is proposed, with the type strain R-40503T ( = R-278T  = LMG 25434T  = CAIM 1459T). The DNA G+C content of strain R-40503T is 46.5 mol%.", }