1887

Abstract

A Gram-negative, aerobic bacterium, designated strain R-40503, was isolated from mucus of the reef-builder coral , located in the São Sebastião Channel, São Paulo, Brazil. Phylogenetic analyses revealed that strain R-40503 belongs to the genus . The 16S rRNA gene sequence similarity of R-40503 was above 97 % with the type strains of , , and , and below 97 % with type strains of the other species. Strain R-40503 showed less than 35 % DNA–DNA hybridization (DDH) with the type strains of the phylogenetically closest species, demonstrating that it should be classified into a novel species. Amplified fragment length polymorphism (AFLP), chemotaxonomic and phenotypic analyses provided further evidence for the proposal of a novel species. Concurrently, a close genomic relationship between and was observed. The type strains of these two species showed 78 % DDH and 63 % AFLP pattern similarity. Their phenotypic features were very similar, and their DNA G+C contents were identical (46.3 mol%). Collectively, these data demonstrate unambiguously that is a later heterotypic synonym of . Several phenotypic features can be used to discriminate between species. The novel strain R-40503 is clearly distinguishable from its neighbours. For instance, it shows oxidase and urease activity, utilizes -asparagine and has the fatty acid C 3-OH but lacks C and C. The name sp. nov. is proposed, with the type strain R-40503 ( = R-278  = LMG 25434  = CAIM 1459). The DNA G+C content of strain R-40503 is 46.5 mol%.

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2011-05-01
2019-10-15
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vol. , part 5, pp. 1170 - 1175

Maximum-parsimony phylogenetic tree showing the phylogenetic position of sp. nov. R-40503 based on 16S rRNA gene sequences.

AFLP DNA fingerprints of strain R-40503 and strains of phylogenetically related species.

Cellular fatty acid contents of strain R-40503 and the type strains of phylogenetically related species.

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