A curved, ring-like bacterium, strain AS 1.1761T, isolated from the roots of Spartina anglica, was studied by a polyphasic approach. According to phylogenetic analysis, strain AS 1.1761T belongs to the genus Ancylobacter, with 99·21 % 16S rDNA sequence similarity to Ancylobacter aquaticus, the only species described so far in this genus. However, strain AS 1.1761T had no significant DNA–DNA binding with the type strain of A. aquaticus. In addition, strain AS 1.1761T differed from A. aquaticus in many phenotypic features. Based on molecular and phenotypic data, a novel species, Ancylobacter rudongensis sp. nov., is proposed. The type strain is AS 1.1761T (=JCM 11671T).
ClausD.,
BergendahlJ. E.,
MandelM.1968; DNA base composition of Microcyclus species and organisms of similar morphology. Arch Mikrobiol 63:26–28[CrossRef]
DoetschR. N.1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp 21–33 Edited by
GerhardtP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.
Washington, DC: American Society for Microbiology;
FarmerJ. J.III,
AsburyM. A.,
HickmanF. W.,
BrennerD. J.the Enterobacteriaceae study group1980; Enterobacter sakazakii : a new species of “ Enterobacteriaceae ” isolated from clinical specimens. Int J Syst Bacteriol 30:569–584[CrossRef]
HansonR. S.,
PhillipsJ. A.1981; Chemical composition. In Manual of Methods for General Bacteriology pp 328–364 Edited by
GerhardtP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.
Washington, DC: American Society for Microbiology;
HoltJ. G.,
KriegN. R.,
SneathP. H. A.,
StaleyJ. T.,
WilliamsS. T.1994; Nonmotile (or rarely motile), Gram-negative curved bacteria. In Bergey's Manual of Determinative Bacteriology , 9th edn. pp 65–69 Edited by
HoltJ. G.,
KriegN. R.,
SneathP. H. A.,
StaleyJ. T.,
WilliamsS. T.
Baltimore: Williams & Wilkins;
HughR.,
LeifsonE.1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J Bacteriol 66:24–26
LapageS. P.,
SneathP. H. A.,
LesselE. F.,
SkermanV. B. D.,
SeeligerH. P. R.,
ClarkW. A.
(editors) 1975International Code of Nomenclature of Bacteria (1976 Revision) . Bacteriological Code Washington, DC: American Society for Microbiology;
LarkinJ. M.,
WilliamsP. M.,
TaylorR.1977; Taxonomy of the genus Microcyclus Ørskov 1928: reintroduction and emendation of the genus Spirosoma Migula 1894 and proposal of a new genus, Flectobacillus
. Int J Syst Bacteriol 27:147–156[CrossRef]
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118[CrossRef]
ØrskovJ.1928; Beschreibung eines neuen Mikroben, Microcyclus aquaticus , mit eigentümlicher Morphologie. Zentbl Bakteriol Parasitenkd Infektkrankh Hyg Abt I Orig 107:180–184 (in German
RajH. D.1981; The genus Microcyclus and related bacteria. In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria pp 630–644 Edited by
StarrM. P.,
StolpH.,
TrüperH. G.,
BalowsA.,
SchlegelH. G.
New York: Springer;
RajH. D.1983; Proposal of Ancylobacter gen. nov. as a substitute for the bacterial genus Microcyclus Ørskov 1928. Int J Syst Bacteriol 33:397–398[CrossRef]
RajH. D.,
MaloyS. R.1990; Proposal of Cyclobacterium marinus gen. nov., comb. nov. for a marine bacterium previously assigned to the genus Flectobacillus
. Int J Syst Bacteriol40337–347[CrossRef]
SaccardoP. A.1905Pyrenomyceteae , Dothideaceae , Hyalodidymae , Microcyclus . In Sylloge Fungorum omnium hucusque Cognitorum . Supplementum universale, vol. 17, part VI pp 844–845 Pavia: P. A; Saccardo (in Latin
SmibertR. M.,
KriegN. R.1981; General characterization. In Manual of Methods for General Bacteriology pp 409–443 Edited by
GerhardtP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.
Washington, DC: American Society for Microbiology;
StaleyJ. T.1974; Genus Microcyclus Ørskov 1928, 183. In Bergey's Manual of Determinative Bacteriology
. , 8th edn. pp 214–215 Edited by
BuchananR. E.,
GibbonsN. E.
Baltimore: Williams & Wilkins;
StaleyJ. T.,
KonopkaA. E.1984; Genus Microcyclus Ørskov 1928, 183AL
. In Bergey's Manual of Systematic Bacteriology vol 1 pp 133–135 Edited by
KriegN. R.,
HoltJ. G.
Baltimore: Williams & Wilkins;
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
Van de PeerY.,
De WachterR.1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570