1887

Abstract

Two restricted facultatively methylotrophic strains, designed B and P, were isolated from rice roots. The isolates were strictly aerobic, Gram-negative, asporogenous, mesophilic, neutrophilic, motile rods that multiplied by binary fission and were able to synthesize indole-3-acetate. The cellular fatty acid profiles of the two strains were dominated by C, Cω7 and C 2-OH. The major ubiquinone was Q-8. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol. Cardiolipin (diphosphatidylglycerol) was absent. The two strains assimilated methanol carbon at the level of formaldehyde via the ribulose monophosphate cycle (2-keto-3-deoxy-6-phosphogluconate variant). They lacked α-ketoglutarate dehydrogenase and glutamate dehydrogenase. They assimilated ammonium via the glutamate cycle enzymes glutamine synthetase and glutamate synthase. The DNA G+C contents of strains B and P were 52.5 and 51.5 mol% ( ), respectively. The level of DNA–DNA reassociation between these strains was 78 %, indicating that they belong to one species. Phylogenetic analysis of strain B based on 16S rRNA and methanol dehydrogenase () gene sequences showed a high level of similarity to members of the genus . As the two isolates were clearly distinct from all recognized members of the genus based on phenotypic data and levels of DNA–DNA relatedness (30–46 %), they are considered to represent a novel species, for which the name sp. nov. is proposed; the type strain is B ( = VKM B-1607 = CCUG 59685 = DSM 5898).

Funding
This study was supported by the:
  • , Russian Federation Basic Research , (Award 10-04-00808-a)
  • , Russian State Contract , (Award 14.740.11.0111)
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2012-01-01
2020-07-10
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