1887

Abstract

Eleven psychrophilic bacteria isolated from Antarctic coastal marine environments were subjected to a polyphasic taxonomic study. The isolates were oxidase-positive, halotolerant, Gram-negative, non-motile coccobacilli with a strictly oxidative metabolism. The DNA G+C content ranged from 44 to 47 mol%. DNA–DNA hybridization experiments showed six homology groups, two of them related at the species level to the type strain of , LMG 7203 (70–83 %). The highest DNA relatedness of two other groups to known species was found to the type strain of , LMG 21282 (51–57 %), and no significant similarity was found between type strains and the last two groups. The predominant cellular fatty acids detected were typical of the genus and included 18 : 19, 16 : 17 and 17 : 18. 16S rRNA gene sequence analysis confirmed that the strains isolated belonged to the genus . The results of the study assigned five isolates to , three isolates to and three isolates to novel species. The names sp. nov. (type strain NF11=LMG 21276= CECT 5885) and sp. nov. (type strain NF23=LMG 21280 =CECT 5889) are proposed for these organisms.

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2003-07-01
2019-09-17
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Transformation of auxotrophic mutant ATCC 43117 to prototrophy. DNA–cell auxotroph (ATCC 43117) mixtures grown overnight on heart infusion agar were streaked on sectors of a medium M9A plate and incubated at 20 °C for 3 days. The sector labelled ATCC 43117 corresponds to a non-DNA-treated control. Sectors NF1, NF7, NF8, NF11, NF18, NF19, NF20, NF23, EN1, EN2, EN4, CEC 465 and CECT 468 contained mixtures of the auxotroph ATCC 43117 and the DNA of the strain indicated.

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