1887

Abstract

Microbial growths causing discoloration on the Roman wall paintings of the Servilia tomb at the necropolis of Carmona (Spain) and the medieval wall paintings of the Saint-Catherine chapel at Castle Herberstein (Austria) were investigated and from four different samples, a group of ten strains with similar characteristics was isolated. The isolates were characterized in a polyphasic taxonomic study, including 16S rDNA sequence analysis, (GTG)-PCR genomic fingerprinting, DNA–DNA hybridization, DNA base ratio, fatty acid analysis, morphological and biochemical characterization. The data obtained attribute the isolates to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain LMG 19507 (=DSM 14890).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02452-0
2003-03-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/2/ijs530459.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02452-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Ciferri, O. ( 1999; ). Microbial degradation of paintings. Appl Environ Microbiol 65, 879–885.
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Gonzalez, I., Laiz, L., Hermosin, B., Caballero, B., Incerti, C. & Saiz-Jimenez, C. ( 1999; ). Bacteria isolated from rock art paintings: the case of Atlanterra shelter (south Spain). J Microbiol Methods 36, 123–127.[CrossRef]
    [Google Scholar]
  5. Goto, K., Omura, T., Hara, Y. & Sadaie, Y. ( 2000; ). Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus. J Gen Appl Microbiol 46, 1–8.[CrossRef]
    [Google Scholar]
  6. Gurtner, C., Heyrman, J., Piñar, G., Lubitz, W., Swings, J. & Rölleke, S. ( 2000; ). Comparative analyses of the bacterial diversity on two different biodeteriorated wall paintings by DGGE and 16S rDNA sequence analysis. Int Biodeterior Biodegrad 46, 229–239.[CrossRef]
    [Google Scholar]
  7. Heyndrickx, M., Vauterin, L., Vandamme, P., Kersters, K. & De Vos, P. ( 1996; ). Applicability of combined amplified ribosomal DNA restriction analysis (ARDRA) patterns in bacterial phylogeny and taxonomy. J Microbiol Methods 26, 247–259.[CrossRef]
    [Google Scholar]
  8. Heyndrickx, M., Lebbe, L., Vancanneyt, M. & 7 other authors ( 1997; ). A polyphasic reassessment of the genus Aneurinibacillus, reclassification of Bacillus thermoaerophilus (Meier-Stauffer et al. 1996) as Aneurinibacillus thermoaerophilus comb. nov., and emended descriptions of A. aneurinilyticus corrig., A. migulanus, and A. thermoaerophilus. Int J Syst Bacteriol 47, 808–817.[CrossRef]
    [Google Scholar]
  9. Heyrman, J. & Swings, J. ( 2001; ). 16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the Servilia tomb (necropolis of Carmona, Seville, Spain). Syst Appl Microbiol 24, 417–422.[CrossRef]
    [Google Scholar]
  10. Heyrman, J., Mergaert, J., Denys, R. & Swings, J. ( 1999; ). The use of fatty acid methyl ester analysis (FAME) for the identification of heterotrophic bacteria present on three mural paintings showing severe damage by microorganisms. FEMS Microbiol Lett 181, 55–62.[CrossRef]
    [Google Scholar]
  11. Karpovich-Tate, N. & Rebrikova, N. L. ( 1990; ). Microbial communities on damaged frescoes and building materials in the cathedral of the nativity of the virgin in the Pafnutii-Borovskii monastery, Russia. Int Biodeterior 27, 281–296.
    [Google Scholar]
  12. Logan, N. A. & Berkeley, R. C. W. ( 1984; ). Identification of Bacillus strains using the API system. J Gen Microbiol 130, 1871–1882.
    [Google Scholar]
  13. Logan, N. A., Lebbe, L., Hoste, B. & 7 other authors ( 2000; ). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50, 1741–1753.
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Nick, G., Jussila, M., Hoste, B., Niemi, R. M., Kaijalainen, S., de Lajudie, P., Gillis, M., de Bruijn, F. J. & Lindström., K. ( 1999; ). Rhizobia isolated from root nodules of tropical leguminous trees characterized using DNA-DNA dot-blot hybridisation and rep-PCR genomic fingerprinting. Syst Appl Microbiol 22, 287–299.[CrossRef]
    [Google Scholar]
  16. Nielsen, P., Fritze, D. & Priest, F. G. ( 1995; ). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745–1761.[CrossRef]
    [Google Scholar]
  17. Pearson, W. R. ( 1994; ). Rapid and sensitive sequence comparison with fast and fasta. Methods Enzymol 193, 63–98.
    [Google Scholar]
  18. Rademaker, J. L. W. & de Bruijn, F. J. ( 1997; ). Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. In DNA Markers: Protocols, Applications and Overviews, pp. 151–171. Edited by G. Gaetano-Anollés & P. M. Gresshoff. New York: Wiley.
  19. Rademaker, J. L. W., Hoste, B., Louws, F. J., Kersters, K., Swings, J., Vauterin, L., Vauterin, P. & de Bruijn, F. J. ( 2000; ). Comparison of AFLP and rep-PCR genomic fingerprinting with DNA–DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 50, 665–677.[CrossRef]
    [Google Scholar]
  20. Rölleke, S., Muyzer, G., Wawer, C., Wanner, G. & Lubitz, W. ( 1996; ). Identification of bacteria in a biodegraded wall painting by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA. Appl Environ Microbiol 62, 2059–2065.
    [Google Scholar]
  21. Saiz-Jimenez, C. & Laiz, L. ( 2000; ). Occurrence of halotolerant/halophilic bacterial communities in deteriorated monuments. Int Biodeterior Biodegrad 46, 319–326.[CrossRef]
    [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  23. Versalovic, J., Schneider, M., de Bruijn, F. J. & Lupksi, J. R. ( 1994; ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40.
    [Google Scholar]
  24. Weirich, G. ( 1989; ). Untersuchungen über Mikroorganismen von Wandmalereien. Mater Org 24, 139–159.
    [Google Scholar]
  25. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02452-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02452-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 459 - 463

Phylogenetic position of LMG 19507 among closely related species, and the type species of related genera based on neighbour joining. Bootstrap values (expressed as percentages of 1000 replications) of 70 % or higher are shown at branch-points. Accession numbers are given in parentheses. Bar, 5 % sequence dissimilarity. [PDF](15 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error