1887

Abstract

Three clusters of isolates have previously been defined within the species , on the basis of DNA–rRNA and DNA–DNA hybridization data, and of protein electrophoretic patterns and immunotyping. More detailed characterization in the current study shows that representatives of these three groups can also be differentiated phenotypically from each other. Strains of ( DNA group 1) are pyrrolidone aminopeptidase-positive, do not grow at 40 °C, are -alanine-positive and are always negative for 4-hydroxybenzoate. Strains of DNA groups 2 and 3 are pyrrolidone aminopeptidase-negative; the former is the only group that is tyrosine-negative, and only the latter can grow at 42 °C (with an optimal growth temperature of 40 °C). These findings are corroborated by differences in 16S rDNA sequence and tRNA intergenic spacer lengths. Therefore, it is proposed to rename DNA group 2 [containing former and E. Falsen (EF) group 10 strains] as sp. nov., and DNA group 3 (containing former EF group 10 strains) as sp. nov.

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2003-05-01
2024-12-12
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