1887

Abstract

Three clusters of isolates have previously been defined within the species , on the basis of DNA–rRNA and DNA–DNA hybridization data, and of protein electrophoretic patterns and immunotyping. More detailed characterization in the current study shows that representatives of these three groups can also be differentiated phenotypically from each other. Strains of ( DNA group 1) are pyrrolidone aminopeptidase-positive, do not grow at 40 °C, are -alanine-positive and are always negative for 4-hydroxybenzoate. Strains of DNA groups 2 and 3 are pyrrolidone aminopeptidase-negative; the former is the only group that is tyrosine-negative, and only the latter can grow at 42 °C (with an optimal growth temperature of 40 °C). These findings are corroborated by differences in 16S rDNA sequence and tRNA intergenic spacer lengths. Therefore, it is proposed to rename DNA group 2 [containing former and E. Falsen (EF) group 10 strains] as sp. nov., and DNA group 3 (containing former EF group 10 strains) as sp. nov.

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2003-05-01
2019-10-15
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References

  1. Baele, M., Baele, P., Vaneechoutte, M., Storms, V., Butaye, P., Devriese, L. A., Verschraegen, G., Gillis, M. & Haesebrouck, F. ( 2000; ). Application of tDNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 38, 4201–4207.
    [Google Scholar]
  2. Baele, M., Storms, V., Haesebrouck, F., Devriese, L. A., Gillis, M., Verschraegen, G., de Baere, T. & Vaneechoutte, M. ( 2001; ). Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of Streptococcus species. J Clin Microbiol 39, 1436–1442.[CrossRef]
    [Google Scholar]
  3. Chang, Y.-H., Han, J., Chun, J., Lee, K. C., Rhee, M.-S., Kim, Y.-B. & Bae, K. S. ( 2002; ). Comamonas koreensis sp. nov., a non-motile species from wetland in Woopo, Korea. Int J Syst Evol Microbiol 52, 377–381.
    [Google Scholar]
  4. De Vos, P., Kersters, K., Falsen, E., Pot, B., Gillis, M., Segers, P. & De Ley, J. ( 1985; ). Comamonas Davis and Park 1962 gen. nov., nom. rev. emend., and Comamonas terrigena Hugh 1962 sp. nov., nom. rev. Int J Syst Bacteriol 35, 443–453.[CrossRef]
    [Google Scholar]
  5. Etchebehere, C., Errazquin, M. I., Dabert, P., Moletta, R. & Muxí, L. ( 2001; ). Comamonas nitrativorans sp. nov., a novel denitrifier isolated from a denitrifying reactor treating landfill leachate. Int J Syst Evol Microbiol 51, 977–983.[CrossRef]
    [Google Scholar]
  6. Gilligan, P. H. & Whittier, S. ( 1999; ). Burkholderia, Xenotrophomonas, Ralstonia, Brevundimonas, Comamonas and Acidovorax. In Manual of Clinical Microbiology, 7th edn, pp. 526–538. Edited by P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover & R. H. Yolken. Washington, DC: American Society for Microbiology.
  7. Gumaelius, L., Magnusson, G., Pettersson, B. & Dalhammar, G. ( 2001; ). Comamonas denitrificans sp. nov., an efficient denitrifying bacterium isolated from activated sludge. Int J Syst Evol Microbiol 51, 999–1006.[CrossRef]
    [Google Scholar]
  8. Laffineur, K., Janssens, M., Charlier, J., Avesani, V., Wauters, G., & Delmée, M. ( 2002; ). Biochemical and susceptibility tests useful for identification of nonfermenting gram-negative rods. J Clin Microbiol 40, 1085–1087.[CrossRef]
    [Google Scholar]
  9. Lindsay, J. A. & Riley, T. V. ( 1991; ). Susceptibility to desferrioxamine: a new test for the identification of Staphylococcus epidermidis. J Med Microbiol 35, 45–48.[CrossRef]
    [Google Scholar]
  10. Nemec, A., De Baere, T., Tjernberg, I., Vaneechoutte, M., van der Reijden, T. J. K. & Dijkshoorn, L. ( 2001; ). Acinetobacter ursingii sp. nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 51, 1891–1899.[CrossRef]
    [Google Scholar]
  11. Vaneechoutte, M., Rossau, R., De Vos, P. & 7 other authors ( 1992; ). Rapid identification of bacteria of the Comamonadaceae with amplified ribosomal DNA-restriction analysis (ARDRA). FEMS Microbiol Lett 93, 227–233.[CrossRef]
    [Google Scholar]
  12. Vaneechoutte, M., Boerlin, P., Tichy, H.-V., Bannerman, E., Jäger, B. & Bille, J. ( 1998; ). Comparison of PCR-based DNA fingerprinting techniques for the identification of Listeria species and their use for atypical Listeria isolates. Int J Syst Bacteriol 48, 127–139.[CrossRef]
    [Google Scholar]
  13. Vaneechoutte, M., Claeys, G., Steyaert, S., De Baere, T., Peleman, R. & Verschraegen, G. ( 2000; ). Isolation of Moraxella canis from an ulcerated metastatic lymph node. J Clin Microbiol 38, 3870–3871.
    [Google Scholar]
  14. Welsh, J. & McClelland, M. ( 1991; ). Genomic fingerprints produced by PCR with consensus tRNA gene primers. Nucleic Acids Res 19, 861–866.[CrossRef]
    [Google Scholar]
  15. Willems, A., Pot, B., Falsen, E., Vandamme, P., Gillis, M., Kersters, K. & De Ley, J. ( 1991; ). Polyphasic taxonomic study of the emended genus Comamonas: relationship to Aquaspirillum aquaticum, E. Falsen group 10, and other clinical isolates. Int J Syst Bacteriol 41, 427–444.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 859 - 862

Strains used and characteristics tested

16S rDNA sequence similarity (%) of species

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