1887

Abstract

The fluorescent amplified fragment length polymorphism (FAFLP) groups A5 (21 isolates), A8 (6 isolates) and A23 (3 isolates) distinguished in an earlier paper (Thompson , 24, 520–538, 2001) were examined in more depth. These three groups were phylogenetically related to , but DNA–DNA hybridization experiments proved that the three AFLP groups are in fact novel species. Chemotaxonomic and phenotypic analyses further revealed several differences among the 30 isolates and known species. It is proposed to accommodate these isolates in three novel species, namely (type strain LMG 20536; EMBL accession no. AJ316171; G+C content of the type strain 46·0 mol%), (type strain LMG 20546; EMBL accession no. AJ316172; G+C content of the type strain 45·9 mol%) and (type strain LMG 21346; EMBL accession no. AJ316181; G+C content of the type strain 46·6 mol%). These species can be differentiated on the basis of phenotypic features, including fatty acid composition (particularly 14 : 0 iso, 14 : 0 iso 3-OH, 16 : 0 iso, 16 : 0, 17 : 0 and 17 : 1ω8), enzyme activities and utilization and fermentation of various carbon sources.

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2003-01-01
2020-01-26
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References

  1. Acar, J. F. & Goldstein, F. W. ( 1996; ). Disc susceptibility test. In Antibiotics in Laboratory Medicine, 4th edn, pp. 1–51. Edited by V. Lorian. Baltimore: Williams & Wilkins.
  2. Baumann, P., Furniss, A. L. & Lee, J. V. ( 1984; ). Genus I. Vibrio Pacini 1854. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 518–538. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  3. Ben-Haim, Y., Thompson, F. L., Thompson, C. C., Cnockaert, M. C., Hoste, B., Swings, J. & Rosenberg, E. ( 2003; ). Vibrio coralliilyticus sp. nov., a temperature-dependent pathogen of the coral Pocillopora damicornis. Int J Syst Evol Microbiol 53, 309–315.[CrossRef]
    [Google Scholar]
  4. Bertone, S., Giacomini, M., Ruggiero, C., Piccarolo, C. & Calegari, L. ( 1996; ). Automated systems for identification of heterotrophic marine bacteria on the basis of their fatty acid composition. Appl Environ Microbiol 62, 2122–2132.
    [Google Scholar]
  5. Eilers, H., Pernthaler, J. & Amann, R. ( 2000; ). Succession of pelagic marine bacteria during enrichment: a close look at cultivation-induced shifts. Appl Environ Microbiol 66, 4634–4640.[CrossRef]
    [Google Scholar]
  6. Farmer, J. J., III & Hickman-Brenner, F. W. ( 1992; ). The genera Vibrio and Photobacterium. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, and Applications, 2nd edn, pp. 2952–3011. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer-Verlag.
  7. Gomez-Gil, B., Tron-Mayén, L., Roque, A., Turnbull, J. F., Inglis, V. & Guerra-Flores, A. L. ( 1998; ). Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei. Aquaculture 163, 1–9.[CrossRef]
    [Google Scholar]
  8. Hansen, G. H. & Olafsen, J. A. ( 1999; ). Bacterial interactions in early life stages of marine cold water fish. Microb Ecol 38, 1–26.[CrossRef]
    [Google Scholar]
  9. Hedlund, B. P. & Staley, J. T. ( 2001; ). Vibrio cyclotrophicus sp. nov., a polycyclic aromatic hydrocarbon (PAH)-degrading marine bacterium. Int J Syst Evol Microbiol 51, 61–66.
    [Google Scholar]
  10. Huys, G., Vancanneyt, M., Coopman, R., Janssen, P., Falsen, E., Altwegg, M. & Kersters, K. ( 1994; ). Cellular fatty acid composition as a chemotaxonomic marker for the differentiation of phenospecies and hybridization groups in the genus Aeromonas. Int J Syst Bacteriol 44, 651–658.[CrossRef]
    [Google Scholar]
  11. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  12. Lambert, M. A., Hickman-Brenner, F. W., Farmer, J. J., III & Moss, C. W. ( 1983; ). Differentiation of Vibrionaceae species by their cellular fatty acid composition. Int J Syst Bacteriol 33, 777–792.[CrossRef]
    [Google Scholar]
  13. Macián, M. C., Ludwig, W., Aznar, R., Grimont, P. A. D., Schleifer, K. H., Garay, E. & Pujalte, M. J. ( 2001; ). Vibrio lentus sp. nov., isolated from Mediterranean oysters. Int J Syst Evol Microbiol 51, 1449–1456.
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Mougel, C., Thioulouse, J., Perrière, G. & Nesme, X. ( 2002; ). A mathematical method for determining genome divergence and species delineation using AFLP. Int J Syst Evol Microbiol 52, 573–586.
    [Google Scholar]
  16. Murray, R. G. E., Doetsch, R. N. & Robinow, C. F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  17. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  18. Pujalte, M.-J., Ortigosa, M., Urdaci, M.-C., Garay, E. & Grimont, P. A. D. ( 1993; ). Vibrio mytili sp. nov., from mussels. Int J Syst Bacteriol 43, 358–362.[CrossRef]
    [Google Scholar]
  19. Rademaker, J. L. W., Hoste, B., Louws, F. J., Kersters, K., Swings, J., Vauterin, L., Vauterin, P. & de Bruijn, F. J. ( 2000; ). Comparison of AFLP and rep-PCR genomic fingerprinting with DNA–DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 50, 665–677.[CrossRef]
    [Google Scholar]
  20. Raguénès, G., Christen, R., Guezennec, J., Pignet, P. & Barbier, G. ( 1997; ). Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana. Int J Syst Bacteriol 47, 989–995.[CrossRef]
    [Google Scholar]
  21. Riquelme, C. E., Jorquera, M. A., Rojas, A. I., Avendaño, R. E. & Reyes, N. ( 2001; ). Addition of inhibitor-producing bacteria to mass cultures of Argopecten purpuratus larvae (Lamarck, 1819). Aquaculture 192, 111–119.[CrossRef]
    [Google Scholar]
  22. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  23. Sherr, E. & Sherr, B. ( 2000; ). Marine microbes: an overview. In Microbial Ecology of the Oceans, pp. 13–46. Edited by D. L. Kirchman. New York: Wiley–Liss.
  24. Suantika, G., Dhert, P., Rombaut, G., Vandenberghe, J., De Wolf, T. & Sorgeloos, P. ( 2001; ). The use of ozone in a high density recirculation system for rotifers. Aquaculture 201, 35–49.[CrossRef]
    [Google Scholar]
  25. Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J. ( 2001; ). Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24, 520–538.[CrossRef]
    [Google Scholar]
  26. Thompson, F. L., Abreu, P. C. & Wasielesky, W. ( 2002a; ). Importance of biofilm for water quality and nourishment in intensive shrimp culture. Aquaculture 203, 263–278.[CrossRef]
    [Google Scholar]
  27. Thompson, F. L., Hoste, B., Vandemeulebroecke, K., Engelbeen, K., Denys, R. & Swings, J. ( 2002b; ). Vibrio trachuri Iwamoto et al. 1995 is a junior synonym of Vibrio harveyi (Johnson and Shunk 1936) Baumann et al. 1981. Int J Syst Evol Microbiol 52, 973–976.[CrossRef]
    [Google Scholar]
  28. Vandamme, P., Segers, P., Ryll, M. & 8 other authors ( 1998; ). Pelistega europaea gen. nov., sp. nov., a bacterium associated with respiratory disease in pigeons: taxonomic structure and phylogenetic allocation. Int J Syst Bacteriol 48, 431–440.[CrossRef]
    [Google Scholar]
  29. Verschuere, L., Rombaut, G., Sorgeloos, P. & Verstraete, W. ( 2000; ). Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64, 655–671.[CrossRef]
    [Google Scholar]
  30. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
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