@article{mbs:/content/journal/ijsem/10.1099/ijs.0.02441-0, author = "Hugenholtz, Philip and Huber, Thomas", title = "Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2003", volume = "53", number = "1", pages = "289-293", doi = "https://doi.org/10.1099/ijs.0.02441-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02441-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.", }