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Volume 61, Issue 6

sp. nov., isolated from a pyrene-degrading consortium Free

Abstract

Strain pht-3B was isolated from a pyrene-degrading consortium of an enriched sediment from the Pacific Ocean, collected during the screening of polycyclic aromatic hydrocarbon-degrading bacteria. Cells were Gram-negative, short rods that were motile by means of flagella. Growth was observed at 0–7 % NaCl and 10–41 °C. The isolate was able to reduce nitrate to nitrite, but not to nitrogen. 16S rRNA gene sequence comparisons showed that strain pht-3B was most closely related to NL21 (97.3 % 16S rRNA gene sequence similarity), C115 (97.1 %), J3 (96.8 %) and KY 101 (96.7 %). DNA–DNA hybridization between strain pht-3B and these reference strains revealed 55, 54, 28 and 42 % DNA–DNA relatedness, respectively. The dominant fatty acids were Cω8 cyclo (22.6 %) and summed feature 8 (consisting of Cω7 and/or Cω6; 60.4 %). The G+C content of the chromosomal DNA was 63 mol%. These characteristics were in good agreement with those of members of the genus . According to cell morphology, physiology, fatty acid composition, 16S rRNA gene sequence analysis and DNA–DNA relatedness, the isolate belonged to the genus but could be readily distinguished from recognized species of the genus. Therefore a novel species is proposed to accommodate strain pht-3B, for which the name sp. nov. is proposed. The type strain is pht-3B ( = CCTCC AB 209302 = LMG 25541 = MCCC 1A01024).

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Funding
This study was supported by the:
  • Scientific Research Foundation of Third Institute of Oceanography, SOA (Award 2009065)
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award 2005DKA21209)
  • COMRA program (Award DYXM115-02-2-05)
  • National Natural Science Foundation of China (Award 40906083)
© 2011 IUMS
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2011-06-01
2024-12-03
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1995 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley;
     [Google Scholar]
  2. Coram N. J., Rawlings D. E. 2002; Molecular relationship between two groups of the genus Leptospirillum and the finding that Leptospirillum ferriphilum sp. nov. dominates South African commercial biooxidation tanks that operate at 40 °C. Appl Environ Microbiol 68:838–845 [View Article][PubMed]
     [Google Scholar]
  3. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Science Press; (in Chinese)
    [Google Scholar]
  4. Kang H. S., Yang H. L., Lee S. D. 2009; Nitratireductor kimnyeongensis sp. nov., isolated from seaweed. Int J Syst Evol Microbiol 59:1036–1039 [View Article][PubMed]
     [Google Scholar]
  5. Kim K.-H., Roh S. W., Chang H.-W., Nam Y.-D., Yoon J.-H., Jeon C.-O., Oh H.-M., Bae J.-W. 2009; Nitratireductor basaltis sp. nov., isolated from black beach sand. Int J Syst Evol Microbiol 59:135–138 [View Article][PubMed]
     [Google Scholar]
  6. Labbé N., Parent S., Villemur R. 2004; Nitratireductor aquibiodomus gen. nov., sp. nov., a novel α-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol 54:269–273 [View Article][PubMed]
     [Google Scholar]
  7. Lai Q., Yuan J., Wang B., Sun F., Qiao N., Zheng T., Shao Z. 2009; Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium. Int J Syst Evol Microbiol 59:1579–1582 [View Article][PubMed]
     [Google Scholar]
  8. Lai Q., Wang L., Liu Y., Fu Y., Zhong H., Wang B., Chen L., Wang J., Sun F., Shao Z. 2011a; Alcanivorax pacificus sp. nov., isolated from a deep sea pyrene-degrading consortium. Int J Syst Evol Microbiol 61:1370–1374[PubMed] [CrossRef]
     [Google Scholar]
  9. Lai Q., Yu Z., Yuan J., Sun F., Shao Z. 2011b; Nitratireductor indicus sp. nov., isolated from deep-sea water. Int J Syst Evol Microbiol 61:295–298 [View Article][PubMed]
     [Google Scholar]
  10. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [View Article][PubMed]
     [Google Scholar]
  11. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr A 479:297–306 [View Article][PubMed]
     [Google Scholar]
  12. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967
     [Google Scholar]
  13. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095[PubMed]
     [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  16. Sasser, M. (1997). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  17. Shieh W. Y., Chen Y.-W., Chaw S.-M., Chiu H.-H. 2003; Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53:479–484 [View Article][PubMed]
     [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  19. Tønjum T., Welty D. B., Jantzen E., Small P. L. 1998; Differentiation of Mycobacterium ulcerans, M. marinum, and M. haemophilum: mapping of their relationships to M. tuberculosis by fatty acid profile analysis, DNA–DNA hybridization, and 16S rRNA gene sequence analysis. J Clin Microbiol 36:918–925[PubMed]
     [Google Scholar]
  20. Wang B. J., Lai Q. L., Cui Z. S., Tan T. F., Shao Z. Z. 2008; A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1. Environ Microbiol 10:1948–1963 [View Article][PubMed]
     [Google Scholar]
  21. Wang B., Tan T., Shao Z. 2009; Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 59:1116–1121 [View Article][PubMed]
     [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  23. Yuan J., Lai Q., Wang B., Sun F., Liu X., Du Y., Li G., Gu L., Zheng T., Shao Z. 2009; Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. Int J Syst Evol Microbiol 59:1158–1161 [View Article][PubMed]
     [Google Scholar]
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Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium
Int J Syst Evol Microbiol 61, 1386 (2011); https://doi.org/10.1099/ijs.0.024356-0
/content/journal/ijsem/10.1099/ijs.0.024356-0
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