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Abstract

Strain pht-3B was isolated from a pyrene-degrading consortium of an enriched sediment from the Pacific Ocean, collected during the screening of polycyclic aromatic hydrocarbon-degrading bacteria. Cells were Gram-negative, short rods that were motile by means of flagella. Growth was observed at 0–7 % NaCl and 10–41 °C. The isolate was able to reduce nitrate to nitrite, but not to nitrogen. 16S rRNA gene sequence comparisons showed that strain pht-3B was most closely related to NL21 (97.3 % 16S rRNA gene sequence similarity), C115 (97.1 %), J3 (96.8 %) and KY 101 (96.7 %). DNA–DNA hybridization between strain pht-3B and these reference strains revealed 55, 54, 28 and 42 % DNA–DNA relatedness, respectively. The dominant fatty acids were Cω8 cyclo (22.6 %) and summed feature 8 (consisting of Cω7 and/or Cω6; 60.4 %). The G+C content of the chromosomal DNA was 63 mol%. These characteristics were in good agreement with those of members of the genus . According to cell morphology, physiology, fatty acid composition, 16S rRNA gene sequence analysis and DNA–DNA relatedness, the isolate belonged to the genus but could be readily distinguished from recognized species of the genus. Therefore a novel species is proposed to accommodate strain pht-3B, for which the name sp. nov. is proposed. The type strain is pht-3B ( = CCTCC AB 209302 = LMG 25541 = MCCC 1A01024).

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2011-06-01
2019-10-19
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Transmission electron micrographs of negatively stained cells of strain pht-3B grown on marine agar 2216 for 48 h. Bars, 0.5µm.

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Cellular fatty acid contents of strain pht-3B and the type strains of other species in the genus . [ PDF] 36 KB

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