1887

Abstract

An actinomycete strain, DMKUA 205, was isolated from a soil sample collected from the Sakaerat Biosphere Reserve in Nakhonratchasima Province, Thailand. The novel strain produced short chains of non-motile spores on the tips of long sporophores branching from the vegetative hyphae. The morphological and chemotaxonomic properties of this new isolate corresponded to those of members of the genus . Furthermore, 16S rRNA gene sequence analysis showed that the strain was closely related to members of the genus . Phenotypic properties and DNA–DNA relatedness values differentiated the new strain from its closest phylogenetic relatives 0351M-12 (35–54 % DNA–DNA relatedness) and 0385M-1 (58–65 % relatedness). On the basis of phenotypic, genotypic and phylogenetic data, strain DMKUA 205 could be clearly distinguished from the type strains of and Therefore, strain DMKUA 205 represents a novel species, for which the name sp. nov. is proposed. The type strain is strain DMKUA 205 ( = BCC 11662 = NBRC 102641). In addition, the DNA–DNA hybridization results from this study revealed that is a later synonym of .

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2011-04-01
2020-01-19
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References

  1. Brosius J. , Palmer M. L. , Kennedy P. J. , Noller H. F. . ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75:, 4801–4805. [CrossRef] [PubMed]
    [Google Scholar]
  2. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  3. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the Nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  5. Hasegawa T. , Takizaea M. , Tanida S. . ( 1983; ). A rapid analysis for chemical grouping aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  6. Hayakawa M. , Nonomura H. . ( 1987; ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  7. Hopwood D. A. , Bibb M. J. , Chater K. F. , Kieser T. , Bruton C. J. , Kieser H. M. , Lydiate D. J. , Smith C. P. , Ward J. M. . ( 1985; ). Preparation of chromosomal, plasmid and phage DNA. . In Genetic Manipulation of Streptomyces – a Laboratory Manual, pp. 79–80. Edited by Hopwood D. A. , Bibb M. J. , Chater K. F. , Kieser H. M. , Lydiate D. J. , Smith C. P. , Ward J. M. , Schrempf H. . . Norwich:: John Innes Foundation;.
    [Google Scholar]
  8. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kudo T. , Itoh T. , Miyadoh S. , Shomura T. , Seino A. . ( 1993; ). Herbidospora gen. nov., a new genus of the family Streptosporangiaceae Goodfellow et al. 1990. . Int J Syst Bacteriol 43:, 319–328. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  11. Lechevalier M. P. , Lechevalier H. A. . ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  12. Lechevalier M. P. , DeBievre C. , Lechevalier H. A. . ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  13. Locci R. . ( 1989; ). Streptomycetes and related genera. . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2463–2464. Edited by Williams S. T. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  14. MIDI ( 2002; ). MIS operating manual, version 4.5. Newark, Delaware:: MIDI, Inc;.
    [Google Scholar]
  15. Mikami H. , Ishida Y. . ( 1983; ). Post-column fluorometric detection of reducing sugars in high-performance liquid chromatography using arginine. . Bunseki Kagaku 32:, E207–E210.[CrossRef]
    [Google Scholar]
  16. Minnikin D. E. , Alshamaony L. , Goodfellow M. . ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. . J Gen Microbiol 88:, 200–204.[PubMed] [CrossRef]
    [Google Scholar]
  17. Minnikin D. E. , Collins M. D. , Goodfellow M. . ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95.[CrossRef]
    [Google Scholar]
  18. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  19. Miyadoh M. . ( 2001; ). Gene analysis method. . In Identification Manual of Actinomycetes, pp. 9–19. Edited by Miyadoh S. , Hamada M. , Hotta K. , Kudo T. , Seino A. , Suzuki K. , Yokota A. . . Japan:: Business Center for Academic Societies Japan;.
    [Google Scholar]
  20. Nakagawa Y. , Tamura T. , Kawasaki H. . ( 2001; ). Gene analysis method. . In Identification Manual of Actinomycetes, pp. 83–117. Edited by Miyadoh S. , Hamada M. , Hotta K. , Kudo T. , Seino A. , Suzuki K. , Yokota A. . . Japan:: Business Center for Academic Societies Japan;.
    [Google Scholar]
  21. Saito H. , Miura K. I. . ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef] [PubMed]
    [Google Scholar]
  22. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Newark, Delaware:: Microbial ID, Inc;.
    [Google Scholar]
  24. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  25. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  26. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  27. Tseng M. , Yang S.-F. , Yuan G.-F. . ( 2010; ). Herbidospora yilanensis sp. nov. and Herbidospora daliensis sp. nov., from sediment. . Int J Syst Evol Microbiol 60:, 1168–1172.[CrossRef]
    [Google Scholar]
  28. Uchida K. , Aida K. . ( 1977; ). Acyl type of bacterial cell wall: its simple identification by a colorimetric method. . J Gen Appl Microbiol 23:, 249–260. [CrossRef]
    [Google Scholar]
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