1887

Abstract

Five Gram-negative bacterial strains, oxidase-positive, motile by means of more than one polar flagella, facultative anaerobe, arginine dihydrolase-negative, lysine- and ornithine decarboxylase-positive, sensitive to the vibriostatic agent O/129, were isolated from a flow-through rotifer culture system in Gent, Belgium, and previously characterized by fluorescent amplified fragment length polymorphism. Comparison of the 16S rDNA sequence of strain LMG 21460 indicated close relationships (∼99 % similarity) to , , and . However, DNA hybridization experiments revealed similarity values below 70 % with its closest species and . Additionally, the analysed strains differ from related species by the utilization of melibiose and production of acid from -arabinose and amygdalin. Among the strains analysed, differences were observed in some phenotypic characters, particularly susceptibility to ampicillin, polymyxin B and amikacin, and urease activity. The major fatty acids identified were 16 : 0, 18 : 1ω7, 14 : 0, 12 : 0 3-OH and 18 : 0. sp. nov. is proposed, with type strain LMG 21460 (=CAIM 577); it has a DNA G+C content of 44·5±0·01 mol%.

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2003-01-01
2019-10-13
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References

  1. Alsina, M. & Blanch, A. R. ( 1994; ). A set of keys for biochemical identification of environmental Vibrio species. J Appl Bacteriol 76, 79–85.[CrossRef]
    [Google Scholar]
  2. Bauer, A. W., Kirby, W. M., Sherris, J. C. & Turck, M. ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45, 493–496.
    [Google Scholar]
  3. Baumann, P. & Schubert, R. H. W. ( 1983; ). Vibrionaceae. In Bergey's Manual of Systematic Bacteriology, pp. 516–550. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  4. Bertone, S., Giacomini, M., Ruggiero, C., Piccarolo, C. & Calegari, L. ( 1996; ). Automated systems for identification of heterotrophic marine bacteria on the basis of their fatty acid composition. Appl Environ Microbiol 62, 2122–2132.
    [Google Scholar]
  5. Dorsch, M., Lane, D. & Stackebrandt, E. ( 1992; ). Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences. Int J Syst Bacteriol 42, 58–63.[CrossRef]
    [Google Scholar]
  6. Douillet, P. A. ( 2000; ). Bacterial additives that consistently enhance rotifer growth under synxenic culture conditions. 2. Use of single and multiple bacterial probiotics. Aquaculture 182, 241–248.[CrossRef]
    [Google Scholar]
  7. Farmer, J. J. ( 1980; ). Revival of the name Vibrio vulnificus. Int J Syst Bacteriol 30, 656.[CrossRef]
    [Google Scholar]
  8. Gatesoupe, F. J. ( 1989; ). Further advances in the nutritional and antibacterial treatments of rotifers as food for turbot larvae, Scophthalmus maximus L. In Aquaculture – a Biotechnology in Progress, pp. 721–730. Edited by N. De Pauw, E. Jaspers, H. Ackefors & N. Wilkins. Bredene, Belgium: European Aquaculture Society.
  9. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). MEGA2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 12, 1244–1245.
    [Google Scholar]
  11. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. In Current Methods for Classification and Identification of Microorganisms, pp. 1–67. Edited by R. R. Colwell & R. Grigorova. London: Academic Press.
  12. Logan, N. A., Lebbe, L., Hoste, B. & 7 other authors ( 2000; ). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50, 1741–1753.
    [Google Scholar]
  13. Maidak, B. L., Cole, J. R., Parker, C. T., Jr & 11 other authors ( 1999; ). A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27, 171–173.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Munro, P. D., Barbour, A. & Birkbeck, T. H. ( 1994; ). Comparison of gut bacterial flora of start-feeding larval turbot under different conditions. J Appl Bacteriol 77, 560–566.[CrossRef]
    [Google Scholar]
  16. Murray, R. G. E., Doetsch, R. N. & Robinow, C. F. ( 1994; ). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  17. Osterhout, G. J., Shull, V. H. & Dick, J. D. ( 1991; ). Identification of clinical isolates of gram-negative nonfermentative bacteria by an automated cellular fatty acid identification system. J Clin Microbiol 29, 1822–1830.
    [Google Scholar]
  18. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  19. Reichelt, J. L., Baumann, P. & Baumann, L. ( 1976; ). Study of genetic relationships among marine species of the genera Beneckea and Photobacterium by means of in vitro DNA/DNA hybridization. Arch Microbiol 110, 101–120.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Skjermo, J. & Vadstein, O. ( 1999; ). Techniques for microbial control in the intensive rearing of marine larvae. Aquaculture 177, 333–343.[CrossRef]
    [Google Scholar]
  22. Suantika, G., Dhert, P., Rombaut, G., Vanderberghe, J., De Wolf, T. & Sorgeloos, P. ( 2001; ). The use of ozone in a high density recirculation system for rotifers. Aquaculture 201, 35–49.[CrossRef]
    [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The CLUSTAL_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  24. Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J. ( 2001; ). Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24, 520–538.[CrossRef]
    [Google Scholar]
  25. Verdonck, L., Swings, J., Kersters, K., Dehasque, M., Sorgeloos, P. & Leger, P. ( 1994; ). Variability of the microbial environment of rotifer Brachionis plicatilis and Artemia production systems. J World Aquacult Soc 25, 55–59.[CrossRef]
    [Google Scholar]
  26. Verdonck, L., Grisez, L., Sweetman, E., Minkoff, G., Sorgeloos, P., Ollevier, F. & Swings, J. ( 1997; ). Vibrios associated with routine productions of Brachionus plicatilis. Aquaculture 149, 203–214.[CrossRef]
    [Google Scholar]
  27. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
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