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Abstract

Three Gram-stain-positive actinobacterial strains, designated YM24-125, YM05-1041 and YM12-102, were isolated from marine environments. Phylogenetic analysis based on 16S rRNA gene sequences indicated that they belonged to the suborder and were most closely related to members of the genus with 95.0–98.4 % sequence similarities. Cells of strains YM05-1041 and YM12-102 were rod-shaped, similar to members of the genus , while cells of strain YM24-125 were cocci to short ovoid rods. All strains grew in the absence of NaCl, but tolerated up to 15 % NaCl. The major menaquinone was demethylmenaquinone DMK-9 (H) and the major polar lipids were phosphatidylinositol, diphosphatidylglycerol and phosphatidyglycerol. All strains contained straight-chain, iso-branched and anteiso-branched saturated fatty acids. The DNA G+C contents were 65.3–67.3 mol%. The peptidoglycans of strains YM05-1041 and YM12-102 were the A4β type with ornithine while that of strain YM24-125 contained ornithine and lysine as diamino acids. Phylogenetic analyses and differences in physiological and biochemical characteristics indicated that these three isolates represent novel species within the genus , for which the names sp. nov. (with YM24-125  = NBRC 106266  = KCTC 19747  = MBIC 08349 as the type strain), sp. nov. (with YM05-1041  = NBRC 106264  = KCTC 19746  = MBIC 08346 as the type strain) and sp. nov. (with YM12-102  = NBRC 106265  = KCTC 19745  = MBIC 08347 as the type strain) are proposed. Based on differences in the quinone system, the presence of demethylmenaquinone DMK-9(H) and the pattern of 16S rRNA signatures, the three novel strains and current members of the genus represent a unique family within the suborder . Therefore, the family fam. nov. is also proposed.

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2011-06-01
2019-09-21
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Composition of H medium. [ PDF] (58 KB)

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Mass spectra of total ion current (TIC). The mass spectrometer was set to the negative ion mode with a spray voltage of −3.5 kV and a capillary temperature of 350 °C. HPLC separation was achieved on a diol phase (Lichrospher 100 Diol 5 µl, Agilent Technologies) using a 4×125 mm column. A flow rate of 1 ml min was employed with the followig solvent gradient: 0 min 100 % A, incereasing over 20 min to 35 % A, 65 % B using a concave curvature, followed by 20 min. Eluent A was a mixture of n-hexane/2-propanol/formic acid/ammonia (79 : 20 : 1.2 : 0.04, by volume). Eluent B was a mixture of 2-propanol/water/formic acid/ammonia (88 : 10 : 1.2 : 0.04, by volume). (a) YM24-125 , (b) YM05-1041 , (c) YM12-102 , (d) JCM 12123 . PI, Phosphatidylinositol; PG, Phosphatidylglycerol; DPG, Diphosphatidylglycerol; Unk, unknown polar lipids.

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Transmission electron micrograph of a negatively stained cell of strain YM24-125 . Bar, 0.5 µm.

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