1887

Abstract

A Gram-type-positive, strictly aerobic actinobacterium, designated strain MON 2.2, was isolated from the surface of a sandstone monument. Cells with a coccoid shape, arranged in pairs or clusters, were non-motile and did not produce spores. The 10 closest 16S rRNA gene sequence matches (~95 % similarity) found in the public databases were uncultured actinobacteria, while the closest cultured members indicated a phylogenetic relationship with members of the family (92–95 % similarity). Subsequent phylogenetic analysis placed the new isolate within the radiation of the genera and , but forming an independent branch. Chemotaxonomic markers were consistent with the classification of strain MON 2.2 in the family , amongst the genera containing -diaminopimelic acid in their peptidoglycan. Characteristic fatty acids iso-C and anteiso-C also supported its affiliation to this taxon; however, polar lipid and menaquinone compositions clearly differentiated strain MON 2.2 from other genera in the family. On the basis of these results and additional physiological data obtained in the present study, it is proposed that strain MON 2.2 be classified in a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is MON 2.2 ( = CECT 7672  = DSM 23257  = LMG 25551).

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2011-05-01
2020-01-17
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Electron micrographs of cells of strain MON 2.2 grown on medium for 5 days. (a) Scanning electron micrograph showing cell arrangement. Bar, 1 µm. (b) Transmission electron micrograph of dividing cells. Bar, 500 nm.

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16S rRNA gene sequence-based phylogenetic tree showing the relationship between the sequence of strain MON 2.2 and the 13 sequences found in the databases that showed highest similarity. [PDF](41 KB)

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Polar lipid profile of strain MON 2.2 . DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PGL1, phosphoglycolipid 1; PL1 and PL2, unidentified phospholipids; GL1–GL4, unidentified glycolipids; L1 and L2, unidentified lipids.

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Fatty acid composition of strain MON 2.2 grown on TSBA for 24 h at 28 °C and analysed using the MIDI system. [PDF](34 KB)

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