1887

Abstract

A Gram-type-positive, strictly aerobic actinobacterium, designated strain MON 2.2, was isolated from the surface of a sandstone monument. Cells with a coccoid shape, arranged in pairs or clusters, were non-motile and did not produce spores. The 10 closest 16S rRNA gene sequence matches (~95 % similarity) found in the public databases were uncultured actinobacteria, while the closest cultured members indicated a phylogenetic relationship with members of the family (92–95 % similarity). Subsequent phylogenetic analysis placed the new isolate within the radiation of the genera and , but forming an independent branch. Chemotaxonomic markers were consistent with the classification of strain MON 2.2 in the family , amongst the genera containing -diaminopimelic acid in their peptidoglycan. Characteristic fatty acids iso-C and anteiso-C also supported its affiliation to this taxon; however, polar lipid and menaquinone compositions clearly differentiated strain MON 2.2 from other genera in the family. On the basis of these results and additional physiological data obtained in the present study, it is proposed that strain MON 2.2 be classified in a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is MON 2.2 ( = CECT 7672  = DSM 23257  = LMG 25551).

Funding
This study was supported by the:
  • Junta de Castilla y León (JCyL) (Award SA100A7)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.024257-0
2011-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/5/1098.html?itemId=/content/journal/ijsem/10.1099/ijs.0.024257-0&mimeType=html&fmt=ahah

References

  1. Akasaka H., Ueki A., Hanada S., Kamagata Y., Ueki K. 2003; Propionicimonas paludicola gen. nov., sp. nov., a novel facultatively anaerobic, Gram-positive, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil. Int J Syst Evol Microbiol 53:1991–1998 [View Article][PubMed]
    [Google Scholar]
  2. Atlas R. M. 1993 In Handbook of Microbiological Media p. 394 Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  3. Bae H.-S., Moe W. M., Yan J., Tiago I., da Costa M. S., Rainey F. A. 2006; Propionicicella superfundia gen. nov., sp. nov., a chlorosolvent-tolerant propionate-forming, facultative anaerobic bacterium isolated from contaminated groundwater. Syst Appl Microbiol 29:404–413 [View Article][PubMed]
    [Google Scholar]
  4. Bates R. G., Bower V. E. 1956; Alkaline solutions for pH control. Anal Chem 28:1322–1324 [View Article]
    [Google Scholar]
  5. Cappitelli F., Principi P., Pedrazzani R., Toniolo L., Sorlini C. 2007; Bacterial and fungal deterioration of the Milan Cathedral marble treated with protective synthetic resins. Sci Total Environ 385:172–181 [View Article][PubMed]
    [Google Scholar]
  6. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  7. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp. 21–33 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  9. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  10. Hopwood D. A., Bibb M. J., Chater F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation in Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  13. Luedemann G. M. 1968; Geodermatophilus, a new genus of the Dermatophilaceae (Actinomycetales). J Bacteriol 96:1848–1858[PubMed]
    [Google Scholar]
  14. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160[PubMed]
    [Google Scholar]
  15. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206 [View Article]
    [Google Scholar]
  16. McIlvaine T. C. 1921; A buffer solution for colorimetric comparison. J Biol Chem 49:183–186
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for extracting bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  18. Nakamura K., Hiraishi A., Yoshimi Y., Kawaharasaki M., Masuda K., Kamagata Y. 1995; Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Bacteriol 45:17–22 [View Article][PubMed]
    [Google Scholar]
  19. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77:4844–4846 [View Article]
    [Google Scholar]
  20. Rivas R., Sánchez M., Trujillo M. E., Zurdo-Piñeiro J. L., Mateos P. F., Martínez-Molina E., Velázquez E. 2003; Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree (Ulmus nigra). Int J Syst Evol Microbiol 53:99–103 [View Article][PubMed]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  22. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156 [View Article]
    [Google Scholar]
  23. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  24. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P. 1997; Friedmanniella antarctica gen. nov., sp. nov., an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47:278–283 [View Article][PubMed]
    [Google Scholar]
  25. Shintani T., Liu W. T., Hanada S., Kamagata Y., Miyaoka S., Suzuki T., Nakamura K. 2000; Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge. Int J Syst Evol Microbiol 50:201–207[PubMed] [CrossRef]
    [Google Scholar]
  26. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  27. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  28. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  30. Trujillo M. E., Kroppenstedt R. M., Fernández-Molinero C., Schumann P., Martínez-Molina E. 2007; Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius . Int J Syst Evol Microbiol 57:2799–2804 [View Article][PubMed]
    [Google Scholar]
  31. Urzì C., Realini M. 1998; Colour changes of Noto’s calcareous sandstone as related with its colonization by microorganisms. Int Biodeter Biodegr 42:45–54 [View Article]
    [Google Scholar]
  32. Urzì C., Brusetti L., Salamone P., Sorlini C., Stackebrandt E., Daffonchio D. 2001; Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin. Environ Microbiol 3:471–479 [View Article][PubMed]
    [Google Scholar]
  33. Whiton R. S., Lau P., Morgan S. L., Gilbart J., Fox A. 1985; Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. J Chromatogr A 347:109–120 [View Article][PubMed]
    [Google Scholar]
  34. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813[PubMed]
    [Google Scholar]
  35. Zhi X.-Y., Li W.-J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.024257-0
Loading
/content/journal/ijsem/10.1099/ijs.0.024257-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Supplementary material 4

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error