1887

Abstract

A polyphasic taxonomic study was performed on 22 thermotolerant, aerobic, endospore-forming bacteria from dairy environments. Seventeen isolates were retrieved from raw milk, one from a filter cloth and four from grass, straw or milking equipment. These latter four isolates (R-6546, R-7499, R-7764 and R-7440) were identified as based on DNA–DNA hybridizations (values above 70 % with LMG 18084) but showed discrepancies in characteristics with the original species description, so an emended description of this species is given. According to 16S rRNA gene sequence analysis and DNA–DNA hybridization experiments, the remaining 18 isolates (R-6488, R-28193, R-6491, R-6492, R-7336, R-33367, R-6486, R-6770, R-31288, R-28160, R-26358, R-7632, R-26955, R-26950, R-33520, R-6484, R-26954 and R-7165) represented one single species, most closely related to (93.9 % 16S rRNA gene sequence similarity), for which the name is proposed. Cells were Gram-stain-positive, facultatively anaerobic, endospore-forming rods that grew optimally at 40–50 °C. The cell wall peptidoglycan type of strain R-6488, the proposed type strain, was A1γ based on -diaminopimelic acid. Major fatty acids of the strains were C (28.0 %), iso-C (12.1 %) and iso-C (12.0 %). MK-7 was the predominant menaquinone, and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and some unidentified phospholipids. DNA G+C content was 35.0 mol%. Phenotypic properties allowed discrimination from other thermotolerant species of the genus and supported the description of the novel species , with strain R-6488 ( = LMG 25569  = DSM 23332) as the proposed type strain.

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2011-08-01
2019-08-26
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Polar lipid patterns for strains R-6488 (left) and LMG 18084 (right).

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