Three chitin-degrading strains representing two novel species were isolated from mangrove forests in Okinawa, Japan. The isolates, ABABA23T, ABABA211 and ABABA212T, were Gram-negative, non-spore-forming, strictly aerobic chemo-organotrophs. The novel strains produced Q-8 as the major isoprenoid quinone component. The predominant fatty acids were iso-C15 : 0 and C16 : 0. On the basis of 16S rRNA gene sequence analysis, the isolates were closely affiliated with members of the genus Microbulbifer. The DNA G+C contents of strains ABABA23T and ABABA212T were 57.8 and 60.2 mol%, respectively. DNA–DNA relatedness values between these two strains and Microbulbifer reference strains were significantly lower than 70 %, the generally accepted threshold level below which strains are considered to belong to separate species. Based on differences in taxonomic characteristics, the three isolates represent two novel species of the genus Microbulbifer, for which the names Microbulbifer chitinilyticus sp. nov. (type strain, ABABA212T = JCM 16148T = NCIMB 14577T) and Microbulbifer okinawensis sp. nov. (type strain, ABABA23T = JCM 16147T = NCIMB 14576T; reference strain, ABABA211) are proposed.
BarrowG. I.,
FelthamR. K. A.
(editors) 1993Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [View Article]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
GonzálezJ. M.,
MayerF.,
MoranM. A.,
HodsonR. E.,
WhitmanW. B.1997; Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47:369–376 [View Article][PubMed]
HughR.,
LeifsonE.1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26[PubMed]
JennerjahnT. C.,
IttekkotV.2002; Relevance of mangroves for the production and deposition of organic matter along tropical continental margins. Naturwissenschaften 89:23–30 [View Article][PubMed]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
LaneD. J.1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by
StackebrandtE.,
GoodfellowM.
Chichester: Wiley;
MuzzarelliR. A. A.1997; Human enzymatic activities related to the therapeutic administration of chitin derivatives. Cell Mol Life Sci 53:131–140 [View Article][PubMed]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
TindallB. J.,
Rosselló-MóraR.,
BusseH.-J.,
LudwigW.,
KämpferP.2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
WangC.-S.,
WangY.,
XuX.-W.,
ZhangD.-S.,
WuY.-H.,
WuM.2009; Microbulbifer donghaiensis sp. nov., isolated from marine sediment of the East China Sea. Int J Syst Evol Microbiol 59:545–549 [View Article][PubMed]
YoonJ.-H.,
KimI.-G.,
ShinD.-Y.,
KangK. H.,
ParkY.-H.2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]
YoonJ.-H.,
KimI.-G.,
OhT.-K.,
ParkY.-H.2004; Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54:1111–1116 [View Article][PubMed]
YoonJ.-H.,
JungS. Y.,
KangS. J.,
OhT.-K.2007; Microbulbifer celer sp. nov., isolated from a marine solar saltern of the Yellow Sea in Korea. Int J Syst Evol Microbiol 57:2365–2369[CrossRef]