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Volume 53, Issue 4

gen. nov., sp. nov., a novel psychrophilic organism isolated from an Antarctic Dry Valley lake Free

Abstract

A novel, psychrophilic, Gram-positive bacterium (designated strain LV3) from a lake near the McMurdo Ice Shelf, Antarctica, has been isolated and characterized. This organism formed red-pigmented colonies, had an optimal growth temperature of 18 °C and grew on a variety of media between −2 and 21 °C. Scanning electron micrographs of strain LV3 that showed small rods with unusual bulbous protuberances during all phases of growth were of particular interest. The G+C content of the genomic DNA was approximately 62 mol%. The cell walls contained ornithine as the diamino acid. The major fatty acids were anteiso-C, iso-C and anteiso-C. Cells grown at −2 °C contained significant amounts of anteiso-C. The major menaquinones found in strain LV3 were MK-11 and MK-12. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain LV3 was a member of the family and related to, but distinct from, organisms belonging to the genera , and . In addition, alignments of 16S rRNA sequences showed that the sequence of strain LV3 contained a 13 bp insertion that was found in only a few related sequences. Based on the low growth temperature, unusual cell shape, distinct 16S rRNA gene sequence and structure and cell-wall amino acid and menaquinone compositions, gen. nov., sp. nov. is proposed, with the type strain LV3 (=ATCC BAA-534 =CIP 107482).

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Keyword(s): DAB, diaminobutyric acid
SGM
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2003-07-01
2024-04-20
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Rhodoglobus vestalii gen. nov., sp. nov., a novel psychrophilic organism isolated from an Antarctic Dry Valley lake
Int J Syst Evol Microbiol 53, 985 (2003); https://doi.org/10.1099/ijs.0.02415-0
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