1887

Abstract

A halotolerant actinomycete strain, designated XJEEM 11063, was isolated from a salt lake in Xinjiang province, north-western China. Strain XJEEM 11063 grew at pH 6.0–8.0 (optimal growth at pH 7.0), between 10 and 40 °C (optimal growth at 28–37 °C) and at salinities of 0–10 % (w/v) NaCl (optimal growth at 0–5 %, w/v). The peptidoglycan type was A4α, and the whole-cell hydrolysates contained glucose, mannose and arabinose. The major fatty acids were anteiso-C, iso-C and anteiso-C. MK-9(H) was the predominant menaquinone and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids and three unknown glycolipids. The genomic DNA G+C content was 71.8 mol%. The chemotaxonomic properties supported the affiliation of strain XJEEM 11063 to the genus . Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to XLG9A10.2 (98.3 %) and DSM 17134 (97.0 %). However, it had relatively low values for DNA–DNA relatedness with the above strains (56.0 % and 47.5 %, respectively). Thus, on the basis of the results from this study, a novel species, sp. nov., is proposed. The type strain is XJEEM 11063 ( = DSM 21949 = CCTCC AA 208063).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.024091-0
2011-04-01
2019-08-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/974.html?itemId=/content/journal/ijsem/10.1099/ijs.0.024091-0&mimeType=html&fmt=ahah

References

  1. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cui X. L. , Schumann P. , Stackebrandt E. , Kroppenstedt R. M. , Pukall R. , Xu L. H. , Rohde M. , Jiang C. L. . ( 2004; ). Myceligenerans xiligouense gen. nov., sp. nov., a novel hyphae-forming member of the family Promicromonosporaceae . . Int J Syst Evol Microbiol 54:, 1287–1293. [CrossRef] [PubMed]
    [Google Scholar]
  3. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  4. DSMZ (2001). Catalogue of Strains, 7th edn. Braunschweig: Deutsche Sammlung von Mikroorganismen und Zellkulturen. http://www.dsmz.de/microorganisms/main.php?content_id = 35
  5. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 1985; ). Conference limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Fitch W. M. . ( 1971; ). Towards defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  8. Groth I. , Schumann P. , Schütze B. , Gonzalez J. M. , Laiz L. , Suihko M. L. , Stackebrandt E. . ( 2006; ). Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs. . Int J Syst Evol Microbiol 56:, 283–287. [CrossRef] [PubMed]
    [Google Scholar]
  9. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  10. Jahnke K. D. . ( 1992; ). Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD system 2600 spectrophotometer on a PC/XT/AT type personal computer. . J Microbiol Methods 15:, 61–73. [CrossRef]
    [Google Scholar]
  11. Kelly K. L. . ( 1964; ). Inter-Society Color Council-National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  12. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kroppenstedt R. M. . ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  14. Li W.-J. , Xu P. , Schumann P. , Zhang Y.-Q. , Pukall R. , Xu L.-H. , Stackebrandt E. , Jiang C.-L. . ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef] [PubMed]
    [Google Scholar]
  15. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  16. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  20. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  21. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  22. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tang S. K. , Wang Y. , Chen Y. , Lou K. , Cao L.‐L. , Xu L. H. , Li W.-J. . ( 2009; a). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . . Int J Syst Evol Microbiol 59:, 2025–2032. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tang S.-K. , Wang Y. , Lou K. , Mao P.-H. , Xu L.-H. , Jiang C.-L. , Kim C.-J. , Li W.-J. . ( 2009; b). Kocuria halotolerans sp. nov., an actinobacterium isolated from a saline soil in China. . Int J Syst Evol Microbiol 59:, 1316–1320. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tang S.-K. , Zhi X.-Y. , Wang Y. , Wu J.-Y. , Lee J.-C. , Kim C.-J. , Lou K. , Xu L.-H. , Li W.-J. . ( 2010; ). Haloactinobacterium album gen. nov., sp. nov, a halophilic actinobacterium, and proposal of Ruaniaceae fam. nov.. Int J Syst Evol Microbiol 60:, 2113–2119.[CrossRef]
    [Google Scholar]
  26. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  27. Williams S. T. . ( 1967; ). Sensitivity of streptomycetes to antibiotics as a taxonomic character. . J Gen Microbiol 46:, 151–160.[PubMed] [CrossRef]
    [Google Scholar]
  28. Williams S. T. , Goodfellow M. , Alderson G. . ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2463–2468. Edited by Williams S. T. , Sharpe M. E. , Holt J. G. . . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.024091-0
Loading
/content/journal/ijsem/10.1099/ijs.0.024091-0
Loading

Data & Media loading...

vol. , part 4, pp. 974 - 978

IMAGE

. Results of the Biolog tests for strains XJEEM 11063 , XLG9A10.2 and DSM 17134 . [ PDF] 60 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error