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Abstract

A halotolerant actinomycete strain, designated XJEEM 11063, was isolated from a salt lake in Xinjiang province, north-western China. Strain XJEEM 11063 grew at pH 6.0–8.0 (optimal growth at pH 7.0), between 10 and 40 °C (optimal growth at 28–37 °C) and at salinities of 0–10 % (w/v) NaCl (optimal growth at 0–5 %, w/v). The peptidoglycan type was A4α, and the whole-cell hydrolysates contained glucose, mannose and arabinose. The major fatty acids were anteiso-C, iso-C and anteiso-C. MK-9(H) was the predominant menaquinone and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids and three unknown glycolipids. The genomic DNA G+C content was 71.8 mol%. The chemotaxonomic properties supported the affiliation of strain XJEEM 11063 to the genus . Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to XLG9A10.2 (98.3 %) and DSM 17134 (97.0 %). However, it had relatively low values for DNA–DNA relatedness with the above strains (56.0 % and 47.5 %, respectively). Thus, on the basis of the results from this study, a novel species, sp. nov., is proposed. The type strain is XJEEM 11063 ( = DSM 21949 = CCTCC AA 208063).

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833800)
  • National Natural Science Foundation of China (Award 30870005 and 30860002)
  • Key Project of International Cooperation (Award 2007DFB31620)
  • Research Fund for the Doctoral Program of Higher Education
  • International Cooperation Research Program of Yunnan Province (Award 2009AC017)
  • Natural Science Foundation of Xinjiang Province of China (Award 2009211B29)
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2011-04-01
2021-10-17
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