1887

Abstract

An acidophilic, slightly thermophilic bacterium, designated strain NTAP-1, that produces a thermostable extracellular acid collagenase activity with potential industrial applications was isolated from soil of Aoba-yama Park, Sendai, Japan. The temperature range for growth was 40–65 °C, with an optimum at 55 °C, and the pH range for growth was 2·5–6·5, with an optimum at pH 5·5. Analysis of the 16S rDNA sequence of strain NTAP-1 showed that it is most closely related to strains of the genus . Consistently, the major constituents of the cell-membrane lipid of strain NTAP-1 were -alicyclic fatty acids. However, DNA–DNA reassociation studies showed only low similarities (less than 33 %) to any type strain of . On the basis of the phenotypic and genotypic properties, a novel species is proposed, sp. nov., represented by strain NTAP-1 (=JCM 11817 =ATCC BAA-609).

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2003-07-01
2019-12-14
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