1887

Abstract

Bacterial spot disease of lamb's lettuce [ (L.) Laterr.] was first observed in fields in 1991. This new bacterial disease is localized in western France in high-technology field production of lamb's lettuce for the preparation of ready-to-use salad. Nineteen strains isolated in 1992 and 1993 from typical black leaf spots of naturally infected lamb's lettuce were characterized and compared with reference strains of and . The pathogenicity of the 19 strains was confirmed by artificial inoculation. Biochemical and physiological tests, fatty acid profiles, DNA–DNA hybridization and other nucleic acid-based tests were performed. A numerical taxonomic analysis of the 19 lamb's lettuce strains showed a single homogeneous phenon closely related to previously described phytopathogenic taxa of the genus . DNA–DNA hybridization studies showed that the lamb's lettuce strains were 91–100 % related to a representative strain, strain CFBP 4730, and constituted a discrete DNA hybridization group, indicating that they belong to the same novel species. Results from DNA–rRNA hybridization, 16S rRNA sequence analysis and fatty acid analysis studies confirmed that this novel species belongs to the -subclass of the and, more specifically, to the family and the genus . The name sp. nov. is proposed for this novel taxon of phytopathogenic bacteria. The type strain is strain CFBP 4730 (=NCPPB 4283).

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2003-05-01
2019-10-20
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vol. , part 3, pp. 795 - 800

Neighbour-joining tree obtained from 16S rRNA gene sequences. The scale bar represents 1 estimated base substitution per 100 nucleotide positions. Percentages refer to bootstrap values of calculated 100 trees. EMBL or GenBank accession numbers are given in parentheses. Abbreviations: ATCC, American Type Culture Collection, Manassas, VA, USA; CCUG, Culture Collection of the University of Göteborg, Department of Clinical Bacteriology, University of Göteborg, Sweden; CFBP, Collection Française de Bactéries Phytopathogènes, Angers, France; DSM, Deutsche Sammlung Von Mikroorganismen und Zellkulturen, Germany; IAM, Institute of Applied Microbiology, University of Tokyo, Japan; LMG, Laboratorium voor Microbiologie, Ghent, Belgium; NCIMB, National Collections of Industrial, Food and Marine Bacteria, Aberdeen, UK; NCPPB, National Collection of Plant-Pathogenic Bacteria, Harpenden, UK. [PDF](31 KB)



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