1887

Abstract

Gram-negative anaerobic rods were isolated from chinchilla () faeces and three strains, ST170, ST180 and ST28, were investigated taxonomically. On the basis of phylogenetic analyses and specific phenotypic characteristics, the three strains belonged to the genus . Phylogenetic analysis of their 16S rRNA gene sequences revealed that strains ST170 and ST180 formed a single cluster and a distinct line of descent. Strain ST170 exhibited 99.7 % 16S rRNA gene sequence similarity with strain ST180 and 95.1, 94.6 and 94.4 % 16S rRNA gene sequence similarity with JCM 13223, JCM 13471 and JCM 5826, respectively. Strain ST28 also formed a distinct line of descent and exhibited the highest 16S rRNA gene sequence similarity with JCM 5828 (98.1 %). Low DNA–DNA relatedness (1 %) between strain ST28 and JCM 5828 clearly indicated that they belonged to different species. Analysis of sequences also supported these relationships. The DNA G+C contents of strains ST170 and ST28 were 45.2 and 41.0 mol%, respectively On the basis of phenotypic characteristics and phylogenetic data, two novel species, sp. nov. (type strain ST170  = JCM 16497  = CCUG 59335) and sp. nov. (type strain ST28  = JCM 16496  = CCUG 59334), are proposed.

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2011-04-01
2019-10-20
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Supplements

vol. , part 4, pp. 877 - 881

Neighbour-joining phylogenetic tree based on sequences showing relationships between strains ST170 , ST180 and ST28 and members of related species.

Phenotypic characteristics of strains of sp. nov. and sp. nov. and their closest phylogenetic neighbours.

Cellular fatty acid compositions of strains of sp. nov. and sp. nov. and their closest phylogenetic neighbours.

[PDF file of Supplementary Figure and Tables](106 KB)



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