1887

Abstract

A filamentous actinomycete, designated strain CCNWNQ 0016, was isolated from soil from a zinc and copper mine in Shaanxi province, north-western China. A polyphasic taxonomic study was carried out to establish the status of strain CCNWNQ 0016. Chemical and morphological properties of the isolate were similar to those of species of the genus . Analysis of the almost complete 16S rRNA gene sequence placed strain CCNWNQ 0016 in the genus where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarities to NBRC 15428 (98.8 %), NBRC 12874 (98.7 %) and NBRC 12842 (98.7 %). Furthermore, DNA–DNA hybridization studies between the novel isolate and these three strains showed relatedness values of 49.6±0.6, 47.2±0.9 and 46.9±1.1 %, respectively. A number of phenotypic properties also enabled the isolate to be differentiated from related species of the genus . Therefore, it is proposed that strain CCNWNQ 0016 (=K42 =ACCC 41871 =HAMBI 3107) should be classified as the type strain of a novel species in the genus , sp. nov. The maximum level of resistance to Zn for strain CCNWNQ 0016 is 35 mM.

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2011-03-01
2021-03-09
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References

  1. Abbas A., Edwards C. 1989; Effects of metals on a range of Streptomyces species. Appl Environ Microbiol 55:2030–2035
    [Google Scholar]
  2. Barceloux D. G. 1999; Zinc. J Toxicol Clin Toxicol 37:279–292 [CrossRef]
    [Google Scholar]
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  4. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Guo J. K., Lin Y. B., Zhao M. L., Sun R., Wang T. T., Tang M., Wei G. H. 2009; Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China. Int J Syst Evol Microbiol 59:1326–1330 [CrossRef]
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  10. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  12. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  13. Lechevalier H. A., Lechevalier M. P. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (SIM Special Publication no. 6) pp 277–284 Edited by Dietz A., Thayer D. W. Arlington, VA: Society for Industrial Biology;
    [Google Scholar]
  14. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  15. Lechevalier M. P., Stern A. E., Lechevalier H. A. 1981; Phospholipids in the taxonomy of actinomycetes. Zentralbl Bakteriol Hyg Abt 1 (Suppl. 11):111–116
    [Google Scholar]
  16. Li H. F., Lin Y. B., Guan W. M., Chang J. L., Xu L., Guo J. K., Wei G. H. 2010; Biosorption of Zn(II) by live and dead cells of Streptomyces ciscaucasicus strain CCNWHX 72-14. J Hazard Mater 179:151–159 [CrossRef]
    [Google Scholar]
  17. Lugauskas A., Levinskaitë L., Peèiulytë D., Repeèkienë J., Motuzas A., Vaisvalavièius R., Prosyèevas I. 2005; Effect of copper, zinc and lead acetates on microorganisms in soil. Ekologija 1:61–69
    [Google Scholar]
  18. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  19. Poopal A. C., Laxman R. S. 2009; Studies on biological reduction of chromate by Streptomyces griseus . J Hazard Mater 169:539–545 [CrossRef]
    [Google Scholar]
  20. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  23. Smith L. A., Alleman B. C., Copley-Graves L. 1994; Biological treatment options. In Emerging Technology for Bioremediation of Metals pp 1–12 Edited by Means J. L., Hinchee R. E. New York: Lewis Publishers;
    [Google Scholar]
  24. Thompson G. A., Watling R. J. 1987; Bioaccumulation potential of heterotrophic bacteria for lead, selenium, and arsenic. Bull Environ Contam Toxicol 38:1049–1054 [CrossRef]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  26. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  27. Wani P. A., Khan M. S., Zaidi A. 2007; Effect of metal tolerant plant growth promoting Bradyrhizobium sp. ( vigna ) on growth, symbiosis, seed yield and metal uptake by greengram plants. Chemosphere 70:36–45 [CrossRef]
    [Google Scholar]
  28. Wani P. A., Khan M. S., Zaidi A. 2008; Impact of zinc-tolerant plant growth-promoting rhizobacteria on lentil grown in zinc-amended soil. Agron Sustain Dev 28:449–455 [CrossRef]
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Bacterial Systematics. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  30. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  31. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol 4 pp 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
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