1887

Abstract

A filamentous actinomycete, designated strain CCNWNQ 0016, was isolated from soil from a zinc and copper mine in Shaanxi province, north-western China. A polyphasic taxonomic study was carried out to establish the status of strain CCNWNQ 0016. Chemical and morphological properties of the isolate were similar to those of species of the genus . Analysis of the almost complete 16S rRNA gene sequence placed strain CCNWNQ 0016 in the genus where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarities to NBRC 15428 (98.8 %), NBRC 12874 (98.7 %) and NBRC 12842 (98.7 %). Furthermore, DNA–DNA hybridization studies between the novel isolate and these three strains showed relatedness values of 49.6±0.6, 47.2±0.9 and 46.9±1.1 %, respectively. A number of phenotypic properties also enabled the isolate to be differentiated from related species of the genus . Therefore, it is proposed that strain CCNWNQ 0016 (=K42 =ACCC 41871 =HAMBI 3107) should be classified as the type strain of a novel species in the genus , sp. nov. The maximum level of resistance to Zn for strain CCNWNQ 0016 is 35 mM.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.024018-0
2011-03-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/3/616.html?itemId=/content/journal/ijsem/10.1099/ijs.0.024018-0&mimeType=html&fmt=ahah

References

  1. Abbas, A. & Edwards, C. ( 1989; ). Effects of metals on a range of Streptomyces species. Appl Environ Microbiol 55, 2030–2035.
    [Google Scholar]
  2. Barceloux, D. G. ( 1999; ). Zinc. J Toxicol Clin Toxicol 37, 279–292.[CrossRef]
    [Google Scholar]
  3. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  4. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow, M. & Minnikin, D. E.. London. : Academic Press.
    [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  8. Guo, J. K., Lin, Y. B., Zhao, M. L., Sun, R., Wang, T. T., Tang, M. & Wei, G. H. ( 2009; ). Streptomyces plumbiresistens sp. nov., a lead-resistant actinomycete isolated from lead-polluted soil in north-west China. Int J Syst Evol Microbiol 59, 1326–1330.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  10. Kelly, K. L. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC. : US Government Printing Office.
    [Google Scholar]
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  13. Lechevalier, H. A. & Lechevalier, M. P. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (SIM Special Publication no. 6), pp. 277–284. Edited by Dietz, A. & Thayer, D. W.. Arlington, VA. : Society for Industrial Biology.
    [Google Scholar]
  14. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  15. Lechevalier, M. P., Stern, A. E. & Lechevalier, H. A. ( 1981; ). Phospholipids in the taxonomy of actinomycetes. Zentralbl Bakteriol Hyg Abt 1 ((Suppl. 11), ), 111–116.
    [Google Scholar]
  16. Li, H. F., Lin, Y. B., Guan, W. M., Chang, J. L., Xu, L., Guo, J. K. & Wei, G. H. ( 2010; ). Biosorption of Zn(II) by live and dead cells of Streptomyces ciscaucasicus strain CCNWHX 72-14. J Hazard Mater 179, 151–159.[CrossRef]
    [Google Scholar]
  17. Lugauskas, A., Levinskaitë, L., Peèiulytë, D., Repeèkienë, J., Motuzas, A., Vaisvalavièius, R. & Prosyèevas, I. ( 2005; ). Effect of copper, zinc and lead acetates on microorganisms in soil. Ekologija 1, 61–69.
    [Google Scholar]
  18. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  19. Poopal, A. C. & Laxman, R. S. ( 2009; ). Studies on biological reduction of chromate by Streptomyces griseus. J Hazard Mater 169, 539–545.[CrossRef]
    [Google Scholar]
  20. Rzhetsky, A. & Nei, M. ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10, 1073–1095.
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  23. Smith, L. A., Alleman, B. C. & Copley-Graves, L. ( 1994; ). Biological treatment options. In Emerging Technology for Bioremediation of Metals, pp. 1–12. Edited by Means, J. L. & Hinchee, R. E.. New York. : Lewis Publishers.
    [Google Scholar]
  24. Thompson, G. A. & Watling, R. J. ( 1987; ). Bioaccumulation potential of heterotrophic bacteria for lead, selenium, and arsenic. Bull Environ Contam Toxicol 38, 1049–1054.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  26. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  27. Wani, P. A., Khan, M. S. & Zaidi, A. ( 2007; ). Effect of metal tolerant plant growth promoting Bradyrhizobium sp. (vigna) on growth, symbiosis, seed yield and metal uptake by greengram plants. Chemosphere 70, 36–45.[CrossRef]
    [Google Scholar]
  28. Wani, P. A., Khan, M. S. & Zaidi, A. ( 2008; ). Impact of zinc-tolerant plant growth-promoting rhizobacteria on lentil grown in zinc-amended soil. Agron Sustain Dev 28, 449–455.[CrossRef]
    [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Bacterial Systematics. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  30. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J. ( 1983; ). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813.
    [Google Scholar]
  31. Williams, S. T., Goodfellow, M. & Alderson, G. ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams, S. T., Sharpe, M. E. & Holt, J. G.. Baltimore. : Williams & Wilkins.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.024018-0
Loading
/content/journal/ijsem/10.1099/ijs.0.024018-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error