1887

Abstract

A novel Gram-positive bacterial strain was isolated from forest soil. According to its 16S rRNA sequence, this strain is a deep-rooting member of the class . The 16S rRNA sequence is most closely related (∼94 % identity) to clones of uncultured bacteria detected in different terrestrial environments, while showing only a remote relationship (∼90 % identity or less) to sequences of cultured species. Cells of the first cultured representative of this phylogenetic cluster are small, short rods that are motile by peritrichous flagella, catalase- and oxidase-positive and grow under aerobic conditions. In liquid culture, flagella from different cells can aggregate to form networks, clearly visible under the light microscope. The peptidoglycan contains -diaminopimelic acid and is directly cross-linked (type A1). Mycolic acids are not present. The polar lipids are phosphatidylinositol and an unidentified phospholipid. Menaquinone MK-7(H) was detected as the predominant isoprenoid quinone. Oleic, 14-methylpentadecanoic, hexadecanoic and 6-heptadecenoic acids are the predominant components of the cellular fatty acid profile. The DNA G+C content is 71 mol%. The distinct phylogenetic position and the unusual combination of chemotaxonomic characteristics justify the proposal of a new genus and species, gen. nov., sp. nov., with the type strain ID131577 (=DSM 14684 =JCM 11494).

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2003-03-01
2019-10-23
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