1887

Abstract

A Gram-negative, non-spore-forming, rod-shaped bacterial strain, RL-2, was isolated from seawater of the East Sea in Korea and was subjected to a polyphasic taxonomic study. Strain RL-2 grew optimally at pH 7.5–8.0, at 20 °C and in the absence of NaCl. Phylogenetic trees based on 16S rRNA gene sequence analysis showed that strain RL-2 forms a cluster with IMCC1704 and various uncultured and unidentified gammaproteobacteria. Strain RL-2 exhibited 16S rRNA gene sequence similarity values of 96.1 % to IMCC1704 and 93.7–99.7 % to the uncultured bacterial clones belonging to the cluster and an unidentified gammaproteobacterium. The fatty acid profile of strain RL-2 was similar to that of IMCC1704, but the predominant ubiquinone type (Q-11) of strain RL-2 was different from that (Q-8) of IMCC1704. The DNA G+C content of strain RL-2 was 61.3 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain RL-2 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is RL-2 ( = KCTC 23145  = CCUG 59307).

Funding
This study was supported by the:
  • , 21C Frontier Program of Microbial Genomics and Applications , (Award MG05-0401-2-0)
  • , Support and Application Project of Biological Resources , (Award M10508050004-06N0805-00410)
  • , Ministry of Education, Science and Technology (MEST) of the Republic of Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023994-0
2011-06-01
2020-07-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/6/1382.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023994-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L. 1981; The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes . In The Prokaryotes vol. 2 pp. 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schleger H. Berlin: Springer;
    [Google Scholar]
  2. Bogan B. W., Sullivan W. R., Kayser K. J., Derr K. D., Aldrich H. C., Paterek J. R. 2003; Alkanindiges illinoisensis gen. nov., sp. nov., an obligately hydrocarbonoclastic, aerobic squalane-degrading bacterium isolated from oilfield soils. Int J Syst Evol Microbiol 53:1389–1395 [CrossRef][PubMed]
    [Google Scholar]
  3. Brisou J., Prévot A. R. 1954; [Studies on bacterial taxonomy. X. The revision of species under Acromobacter group]. Ann Inst Pasteur (Paris) 86:722–728 (in French) [PubMed]
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Juni E., Heym G. A. 1986; Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of Gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36:388–391 [CrossRef]
    [Google Scholar]
  7. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  8. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
    [Google Scholar]
  9. Lwoff A. 1939; Révision et démembrement des Hemophilae, le genre Moraxella nov. gen. Ann Inst Pasteur (Paris) 62:168–176 (in French)
    [Google Scholar]
  10. Rossau R., Van Landschoot A., Gillis M., De Ley J. 1991; Taxonomy of Moraxellaceae fam. nov., a new bacterial family to accommodate the genera Moraxella, Acinetobacter, and Psychrobacter and related organisms. Int J Syst Bacteriol 41:310–319 [CrossRef]
    [Google Scholar]
  11. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  12. Song J., Choo Y.-J., Cho J.-C. 2008; Perlucidibaca piscinae gen. nov., sp. nov., a freshwater bacterium belonging to the family Moraxellaceae . Int J Syst Evol Microbiol 58:97–102 [CrossRef][PubMed]
    [Google Scholar]
  13. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  14. Staley J. T., Irgens R. L., Brenner D. J. 1987; Enhydrobacter aerosaccus gen. nov., sp. nov., a gas-vacuolated, facultatively anaerobic, heterotrophic rod. Int J Syst Bacteriol 37:289–291 [CrossRef]
    [Google Scholar]
  15. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  17. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef][PubMed]
    [Google Scholar]
  18. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023994-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023994-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error