1887

Abstract

Two unique psychrophilic strains (CMS 76r and CMS 81y) were isolated from a cyanobacterial mat sample from a pond in Wright Valley, McMurdo, Antarctica. Both isolates were assigned to the genus , since they were Gram-positive, curved rods, non-motile, catalase-positive, contained -2,4-diaminobutyric acid, menaquinone MK-11, phosphatidylglycerol and diphosphatidylglycerol, had a high content of anteiso- and iso-branched fatty acids and had a DNA G+C content of 64–66 mol%. In addition, both isolates were related to the five reported species of at a level of about 95–96 % 16S rDNA sequence similarity and differed from one another by 2·5 %. Strains CMS 76r and CMS 81y also differed from one another in many other phenotypic characteristics and exhibited only 30 % relatedness at the DNA–DNA level, thus indicating that they represent two different species. Furthermore, these two isolates also showed many distinct differences with respect to the reported species of in terms of their phenotypic characteristics, biochemical properties, chemotaxonomic features, sensitivity to various antibiotics and 16S rDNA similarity, clearly indicating that strains CMS 76r (=MTCC 4210 =DSM 15304 =CIP 107783) and CMS 81y (=MTCC 4657 =DSM 15303 =CIP 107785) represent the type strains of two novel species of , for which the names sp. nov. and sp. nov. are proposed.

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2003-07-01
2019-10-16
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UPGMA phenogram showing the phylogenetic relationships between sp. nov. CMS 76r and sp. nov. CMS 81y and other species of the genus and a wider selection of related reference micro-organisms based on 16S rRNA gene sequence analysis. Bootstrap values are given at nodes. Branch lengths are not to scale. [PDF](123 KB)

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Differences between the 16S rDNA sequences of sp. nov. CMS 76r , sp. nov. CMS 81y and other members of the genus . [PDF](113 KB)

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