@article{mbs:/content/journal/ijsem/10.1099/ijs.0.023945-0, author = "Pérez, Tania and Balcázar, José Luis and Peix, Alvaro and Valverde, Angel and Velázquez, Encarna and de Blas, Ignacio and Ruiz-Zarzuela, Imanol", title = "Lactococcus lactis subsp. tructae subsp. nov. isolated from the intestinal mucus of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2011", volume = "61", number = "8", pages = "1894-1898", doi = "https://doi.org/10.1099/ijs.0.023945-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.023945-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "The species Lactococcus lactis currently includes three subspecies; L. lactis subsp. lactis and L. lactis subsp. cremoris, isolated from milk sources, and L. lactis subsp. hordniae, isolated from the leafhopper Hordnia circellata. In this study, three strains, designated L105T, I3 and L101, were isolated from the intestinal mucus of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss). These strains were closely related to members of the species Lactococcus lactis. Strain L105T showed 99.4 % 16S rRNA gene sequence similarity to that of the type strains L. lactis subsp. lactis NCDO 604T and L. lactis subsp. hordniae NCDO 2181T and showed 99.9 % similarity to the type strain Lactococcus lactis subsp. cremoris NCDO 607T. Analysis of two housekeeping genes, rpoB and recA, confirmed the close relationship between the novel strains and L. lactis subsp. cremoris with similarities of 99.3 and 99.7 %, respectively. The three strains could, however, be differentiated from their closest relatives on the basis of several phenotypic characteristics, as was the case for L. lactis subsp. lactis and L. lactis subsp. hordniae, which were also closely related on the basis of 16S rRNA, rpoB and recA gene sequence similarities. The strains isolated in this study represent a new subspecies, for which the name Lactococcus lactis subsp. tructae subsp. nov. is proposed. The type strain is L105T ( = LMG 24662T  = DSM 21502T).", }