1887

Abstract

A bacterial strain, strain IMMIB R-5091, isolated from a cosmetic dye was characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short-chain mycolic acids consistent with the genus . Comparative 16S rRNA gene sequencing showed that the isolate constitutes a distinct subline within the genus , displaying >2·6 % sequence divergence from established species. The isolate could be distinguished from other members of the genus by biochemical tests. Based on both phenotypic and phylogenetic evidence, it is proposed that strain IMMIB R-5091 (=DSM 44530=NRRL B-24142) be classified as the type strain of a novel species, sp. nov.

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2003-05-01
2019-10-17
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References

  1. Becker, B., Lechevalier, M. P., Gordon, R. E. & Lechevalier, H. A. ( 1964; ). Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole cell hydrolysates. Appl Microbiol 12, 421–423.
    [Google Scholar]
  2. Brennan, N. M., Brown, R., Goodfellow, M., Ward, A. C., Beresford, T. P., Simpson, P. J., Fox, P. F. & Cogan, T. M. ( 2001; ). Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese. Int J Syst Evol Microbiol 51, 843–852.[CrossRef]
    [Google Scholar]
  3. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of the 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  5. Collins, M. D., Goodfellow, M. & Minnikin, D. E. ( 1982a; ). A survey of the structures of mycolic acids in Corynabacterium and related taxa. J Gen Microbiol 128, 129–149.
    [Google Scholar]
  6. Collins, M. D., Goodfellow, M. & Minnikin, D. E. ( 1982b; ). Fatty acid composition of some mycolic acid-containing coryneform bacteria. J Gen Microbiol 128, 2503–2509.
    [Google Scholar]
  7. Collins, M. D., Hoyles, L., Hutson, R. A., Foster, G. & Falsen, E. ( 2001; ). Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat. Int J Syst Evol Microbiol 51, 1349–1352.
    [Google Scholar]
  8. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  9. Fernández-Garayzábal, J. F., Collins, M. D., Hutson, R. A., Gonzalez, I., Fernández, E. & Domínguez, L. ( 1998; ). Corynebacterium camporealensis sp. nov., associated with subclinical mastitis in sheep. Int J Syst Bacteriol 48, 463–468.[CrossRef]
    [Google Scholar]
  10. Funke, G., Von Graevenitz, A., Clarridge, J. E., III & Bernard, K. A. ( 1997a; ). Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10, 125–159.
    [Google Scholar]
  11. Funke, G., Lawson, P. A. & Collins, M. D. ( 1997b; ). Corynebacterium mucifaciens sp. nov., an unusual species from human clinical material. Int J Syst Bacteriol 47, 952–957.[CrossRef]
    [Google Scholar]
  12. Funke, G., Lawson, P. A. & Collins, M. D. ( 1998; ). Corynebacterium riegelii sp. nov., an unusual species isolated from female patients with urinary tract infections. J Clin Microbiol 36, 624–627.
    [Google Scholar]
  13. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobic Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  14. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  15. Keddie, R. M. & Cure, G. L. ( 1977; ). The cell wall composition and distribution of free mycolic acids in named strains of coryneform bacteria and in isolates from various natural sources. J Appl Bacteriol 42, 229–252.[CrossRef]
    [Google Scholar]
  16. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  17. Lechevalier, M. P. ( 1968; ). Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71, 934–944.
    [Google Scholar]
  18. Lechevalier, M. P. & Lechevalier, H. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  19. Lechevalier, M. P., de Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  20. Ludwig, W. & Strunk, O. ( 1996; ). arb, a software environment for sequence data. http://www.mikro.biologie.tu-muenchen.de/pub/ARB/documentation/arb.ps
  21. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  22. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–223.[CrossRef]
    [Google Scholar]
  23. Pascual, C., Lawson, P. A., Farrow, J. A. E., Navarro Gimenez, M. & Collins, M. D. ( 1995; ). Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences. Int J Syst Bacteriol 45, 724–728.[CrossRef]
    [Google Scholar]
  24. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  25. Riegel, P., de Briel, D., Prévost, G., Jehl, F. & Monteil, H. ( 1993; ). Proposal of Corynebacterium propinquum sp. nov. for Corynabacterium group ANF-3 strains. FEMS Microbiol Lett 113, 229–234.[CrossRef]
    [Google Scholar]
  26. Riegel, P., Ruimy, R., Renaud, F. N. R., Freney, J., Prevost, G., Jehl, F., Christen, R. & Monteil, H. ( 1997; ). Corynebacterium singulare sp. nov., a new species for urease-positive strains related to Corynebacterium minutissimum. Int J Syst Bacteriol 47, 1092–1096.[CrossRef]
    [Google Scholar]
  27. Ruimy, R., Riegel, P., Boiron, P., Monteil, H. & Christen, R. ( 1995; ). Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences. Int J Syst Bacteriol 45, 740–746.[CrossRef]
    [Google Scholar]
  28. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  29. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  30. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  31. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–471.[CrossRef]
    [Google Scholar]
  32. Wattiau, P., Janssens, M. & Wauters, G. ( 2000; ). Corynebacterium simulans sp. nov., a non-lipophilic, fermentative Corynebacterium. Int J Syst Evol Microbiol 50, 347–353.[CrossRef]
    [Google Scholar]
  33. Yassin, A. F. ( 1988; ). Chemotaxonomic Untersuchungen zur vereinfachten Differenzierung und Identifizierung von aeroben Aktinomyzeten und Mykobakterien. Inaaugural-Dissertation zur Erlangung des Doktorgrades der Methematische-Naturwissenschaftlichen Fakultät der Rheinischen friedrich-Wilhelms-Universität Bonn.
  34. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P. ( 1993; ). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment-mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef]
    [Google Scholar]
  35. Yassin, A. F., Rainey, F. A., Burghardt, J., Brzezinka, H., Mauch, M. & Schaal, K. P. ( 2000; ). Nocardia paucivorans sp. nov. Int J Syst Evol Microbiol 50, 803–809.[CrossRef]
    [Google Scholar]
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