Two psychrophilic strains, Cr7-05T and Cr4-44T, isolated from alpine glacier cryoconite, were characterized by using a polyphasic approach. Both strains were psychrophilic, showing good growth over a temperature range of 1–20 °C. The chemotaxonomic characteristics of these isolates included the presence of C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major cellular fatty acids, Q-10 as the predominant ubiquinone and diphosphatidylglycerol, phosphatidylglycerol and unknown glycolipids as major polar lipids. The DNA G+C contents of strains Cr7-05T and Cr4-44T were 61.4 and 63.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus Devosia. The 16S rRNA gene sequence similarity between the two strains was 98.6 %, but DNA–DNA hybridization indicated 54 % relatedness. Strains Cr7-05T and Cr4-44T exhibited 16S rRNA gene sequence similarity of 94.7–97.2 and 94.9–96.9 %, respectively, to the type strains of recognized Devosia species. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strains Cr7-05T and Cr4-44T represent two novel species within the genus Devosia, for which the names Devosia psychrophila sp. nov. (type strain Cr7-05T = DSM 22950T = CGMCC 1.10210T = CIP 110130T) and Devosia glacialis sp. nov. (type strain Cr4-44T = CGMCC 1.10691T = LMG 26051T) are proposed. An emended description of the genus Devosia is also provided.
BrosiusJ.,
PalmerM. L.,
KennedyP. J.,
NollerH. F.1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli
. Proc Natl Acad Sci U S A 75:4801–4805 [View Article][PubMed]
ChunJ.,
LeeJ.-H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY. W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
CollinsM. D.1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–287 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
De LeyJ.,
CattoirH.,
ReynaertsA.1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
FelsensteinJ.2009; phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
FosterJ. W.1944; Microbiological aspects of riboflavin. I. Introduction. II. Bacterial oxidation of riboflavin to lumichrome. J Bacteriol 47:27–41[PubMed]
HußV. A. R.,
FestlH.,
SchleiferK. H.1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192[CrossRef]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
KumarM.,
VermaM.,
LalR.2008; Devosia chinhatensis sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site in India. Int J Syst Evol Microbiol 58:861–865 [View Article][PubMed]
MargesinR.,
GanderS.,
ZackeG.,
GounotA. M.,
SchinnerF.2003; Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 7:451–458 [View Article][PubMed]
NakagawaY.,
SakaneT.,
YokotaA.1996; Transfer of “Pseudomonas riboflavina” (Foster 1944), a gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev.. Int J Syst Bacteriol 46:16–22 [View Article][PubMed]
ReasonerD. J.,
GeldreichE. E.1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7[PubMed]
RivasR.,
WillemsA.,
Subba-RaoN. S.,
MateosP. F.,
DazzoF. B.,
KroppenstedtR. M.,
Martínez-MolinaE.,
GillisM.,
VelázquezE.2003; Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans, an aquatic legume from India. Syst Appl Microbiol 26:47–53 [View Article][PubMed]
SasserM.1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
VanniniC.,
RosatiG.,
VerniF.,
PetroniG.2004; Identification of the bacterial endosymbionts of the marine ciliate Euplotes magnicirratus (Ciliophora, Hypotrichia) and proposal of ‘Candidatus Devosia euplotis’. Int J Syst Evol Microbiol 54:1151–1156 [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.
other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
YoonJ.-H.,
KangS.-J.,
ParkS.,
OhT.-K.2007; Devosia insulae sp. nov., isolated from soil, and emended description of the genus Devosia
. Int J Syst Evol Microbiol 57:1310–1314 [View Article][PubMed]
ZhangD.-C.,
RedzicM.,
SchinnerF.,
MargesinR.2011; Glaciimonas immobilis gen. nov., sp. nov., a member of the family Oxalobacteraceae isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 61:2186–2190 [View Article][PubMed]