1887

Abstract

Two psychrophilic strains, Cr7-05 and Cr4-44, isolated from alpine glacier cryoconite, were characterized by using a polyphasic approach. Both strains were psychrophilic, showing good growth over a temperature range of 1–20 °C. The chemotaxonomic characteristics of these isolates included the presence of Cω7 and summed feature 3 (Cω7 and/or Cω6) as the major cellular fatty acids, Q-10 as the predominant ubiquinone and diphosphatidylglycerol, phosphatidylglycerol and unknown glycolipids as major polar lipids. The DNA G+C contents of strains Cr7-05 and Cr4-44 were 61.4 and 63.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus . The 16S rRNA gene sequence similarity between the two strains was 98.6 %, but DNA–DNA hybridization indicated 54 % relatedness. Strains Cr7-05 and Cr4-44 exhibited 16S rRNA gene sequence similarity of 94.7–97.2 and 94.9–96.9 %, respectively, to the type strains of recognized species. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strains Cr7-05 and Cr4-44 represent two novel species within the genus , for which the names sp. nov. (type strain Cr7-05  = DSM 22950  = CGMCC 1.10210  = CIP 110130) and sp. nov. (type strain Cr4-44  = CGMCC 1.10691  = LMG 26051) are proposed. An emended description of the genus is also provided.

Funding
This study was supported by the:
  • , Aktion D. Swarovski & Co. 2009
  • , University of Innsbruck
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2012-03-01
2021-03-05
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