@article{mbs:/content/journal/ijsem/10.1099/ijs.0.023929-0, author = "Hahn, Martin W. and Lang, Elke and Brandt, Ulrike and Spröer, Cathrin", title = "Polynucleobacter acidiphobus sp. nov., a representative of an abundant group of planktonic freshwater bacteria", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2011", volume = "61", number = "4", pages = "788-794", doi = "https://doi.org/10.1099/ijs.0.023929-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.023929-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "The heterotrophic, aerobic, facultatively anaerobic, catalase- and oxidase-positive, non-motile strain MWH-PoolGreenA3T, isolated from a rock pool filled with freshwater, was characterized. The strain grew on NSY medium over a NaCl range of 0.0–0.3 % (w/v). Whole-cell fatty acids were dominated by C16 : 1ω7c (feature 3), C18 : 1ω7c and straight-chain C16 : 0; furthermore, the components C12 : 0 and C14 : 0 2-OH were present. The DNA G+C content was 48.3 mol%. Phylogenetic analysis as well as strong similarities in phenotypic and chemotaxonomic traits indicated the affiliation with the genus Polynucleobacter. 16S rRNA gene similarity values with the three described species of the genus Polynucleobacter ranged from 96.7 to 97.8 %. DNA–DNA hybridization experiments did not reveal that the strain belongs to a previously described species of the genus Polynucleobacter. The strain can be discriminated from previously established species of the genus Polynucleobacter by chemotaxonomic and phenotypic traits. The bacterium possesses a free-living lifestyle and represents a group of planktonic freshwater bacteria occurring with high cell numbers in many freshwater lakes. Based on the phylogeny revealed and the chemotaxonomic and phenotypic differences from previously described species of the genus Polynucleobacter, we propose to establish the novel species Polynucleobacter acidiphobus sp. nov. with the type strain MWH-PoolGreenA3T ( = DSM 21994T  = CIP 110079T).", }