A Gram-positive-staining, aerobic, moderately halophilic bacterium, designated strain Y32T, was isolated from subsurface soil of the Sanwei salt field, Yancheng, Jiangsu province, South-east China. The cell-wall peptidoglycan type of strain Y32T was meso-diaminopimelic acid. The menaquinone was menaquinone-7 (MK-7). The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0 and the DNA G+C content of strain Y32T was 40.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y32T was closely related to the type strains of the genus Pontibacillus. The 16S rRNA gene sequence similarities of strain Y32T with the type strains of species of the genus Pontibacillus were 97.8 % (Pontibacillus marinus KCTC 3917T), 96.9 % (Pontibacillus chungwhensis BH030062T), 96.8 % (Pontibacillus litoralis JSM 072002T) and 96.0 % (Pontibacillus halophilus JSM 076056T). DNA–DNA relatedness between strain Y32T and P. marinus KCTC 3917T was 42 %. On the basis of phenotypic, chemotaxonomic and phylogenetic evidences, strain Y32T was found to be affiliated to the genus Pontibacillus, but was clearly differentiated from other members of this genus. Strain Y32T represents a novel member of the genus, for which the name Pontibacillus yanchengensis sp. nov. is proposed. The type strain is Y32T ( = CGMCC 1.10680T = CCTCC AB209311T = NRRL B-59408T).
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 30671140)
AshC.,
FarrowJ. A. E.,
WallbanksS.,
CollinsM. D.1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13:202–206 [View Article]
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
ChenY.-G.,
ZhangY.-Q.,
YiL.-B.,
LiZ.-Y.,
WangY.-X.,
XiaoH.-D.,
ChenQ.-H.,
CuiX.-L.,
LiW.-J.2010; Pontibacillus litoralis sp. nov., a facultatively anaerobic bacterium isolated from a sea anemone, and emended description of the genus Pontibacillus
. Int J Syst Evol Microbiol 60:560–565 [View Article][PubMed]
ChunJ.,
LeeJ.-H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY. W.2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
FairleyD. J.,
BoydD. R.,
SharmaN. D.,
AllenC. C. R.,
MorganP.,
LarkinM. J.2002; Aerobic metabolism of 4-hydroxybenzoic acid in Archaea via an unusual pathway involving an intramolecular migration (NIH shift). Appl Environ Microbiol 68:6246–6255 [View Article][PubMed]
FelsensteinJ.2002; phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington; Seattle, USA:
HußV. A. R.,
FestlH.,
SchleiferK. H.1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192[CrossRef]
LechevalierM. P.,
LechevalierH. A.1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Special Publication no. 6) pp. 227–291 Edited by
DietzA.,
ThayerJ.
Arlington, VA: Society for Industrial Microbiology;
LimJ.-M.,
JeonC. O.,
SongS. M.,
KimC.-J.2005a; Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55:165–170 [View Article][PubMed]
LimJ.-M.,
JeonC. O.,
ParkD.-J.,
KimH.-R.,
YoonB.-J.,
KimC.-J.2005b; Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus
. Int J Syst Evol Microbiol 55:1027–1031 [View Article][PubMed]
LoganN. A.,
BergeO.,
BishopA. H.,
BusseH.-J.,
De VosP.,
FritzeD.,
HeyndrickxM.,
KämpferP.,
RabinovitchL. et al.2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121 [View Article][PubMed]
MataJ. A.,
Martínez-CánovasJ.,
QuesadaE.,
BéjarV.2002; A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [View Article][PubMed]
NielsenP.,
RaineyF. A.,
OuttrupH.,
PriestF. G.,
FritzeD.1994; Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus
. FEMS Microbiol Lett 117:61–66 [View Article]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.1994; clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
XieC. H.,
YokotaA.2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]