1887

Abstract

The term ‘flexispira’ refers to micro-organisms with a particular morphology: fusiform-shaped with helical periplasmic fibrils and bipolar tufts of sheathed flagella. Two flexispira taxa have been formally named, and , a third named species is and eight additional 16S rRNA sequence-based flexispira taxa have been described by Dewhirst . ( 50, 1781–1787, 2000) and given the provisional designation sp. flexispira taxa 1–5, 7, 8 and 10. In the present study, seven gastric or intestinal flexispira isolates from seven Finnish pigs originating from different farms were characterized. Morphologically, all these porcine isolates had typical flexispira morphology. Analysis of the 16S rDNA sequences of five isolates showed that they were most closely related to the sequences of flexispira taxa 4 and 5 and (taxon 6), but less closely related to taxa 1–3 and 8, and . Phenotypic characterization, analysis of RFLPs of 16S and 23S rDNAs and SDS-PAGE profiles revealed that all of the porcine isolates, reference strains of flexispira taxa 1, 4 and 5 and the type strain of (ATCC 700114) had highly related characteristics that differed from those of the reference strains of taxa 2, 3 and 8 and . Furthermore, a high DNA–DNA binding rate was found, in dot-blot hybridization studies, between the Finnish porcine strains, taxa 1, 4 and 5 reference strains and ATCC 700114. In conclusion, polyphasic characterization of novel porcine flexispira isolates and previously described taxa 1, 4 and 5 reference strains showed that they all belong to a validly described species, , and that the taxonomy of known flexispiras is less complicated than proposed on the basis of 16S rDNA sequence analysis.

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2003-03-01
2019-10-22
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References

  1. Archer, J. R., Romero, S., Ritchie, A. E., Hamacher, M. E., Steiner, B. M., Bryner, J. H. & Schell, R. F. ( 1988; ). Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. J Clin Microbiol 26, 101–105.
    [Google Scholar]
  2. Aspinall, S. T., Wareing, D. R. A., Hayward, P. G. & Hutchinson, D. N. ( 1996; ). A comparison of a new campylobacter selective medium (CAT) with membrane filtration for the isolation of thermophilic campylobacters including Campylobacter upsaliensis. J Appl Bacteriol 80, 645–650.[CrossRef]
    [Google Scholar]
  3. Bryner, J. H. ( 1988; ). Flexispira rappini, gen. nov., sp. nov. A motile, urease-producing rod similar to Campylobacter pyloridis. In Campylobacter IV. Proceedings of the Fourth International Workshop on Campylobacter Infections, pp. 440–442. Edited by B. Kaijser & E. Falsen. Göteberg: Göterna Kungälv.
  4. Bryner, J. H., Ritchie, A. E., Pollet, L., Kirkbride, C. A. & Collins, J. E. ( 1987; ). Experimental infection and abortion of pregnant guinea pigs with a unique spirillum-like bacterium isolated from aborted ovine fetuses. Am J Vet Res 48, 91–95.
    [Google Scholar]
  5. Dewhirst, F. E., Chien, C. C., Paster, B. J., Ericson, R. L., Orcutt, R. P., Schauer, D. B. & Fox, J. G. ( 1999; ). Phylogeny of the defined murine microbiota: altered Schaedler flora. Appl Environ Microbiol 65, 3287–3292.
    [Google Scholar]
  6. Dewhirst, F. E., Fox, J. G., Mendes, E. N., Paster, B. J., Gates, C. E., Kirkbride, C. A. & Eaton, K. A. ( 2000a; ).Flexispira rappini’ strains represent at least 10 Helicobacter taxa. Int J Syst Evol Microbiol 50, 1781–1787.
    [Google Scholar]
  7. Dewhirst, F. E., Fox, J. G. & On, S. L. W. ( 2000b; ). Recommended minimal standards for describing new species of the genus Helicobacter. Int J Syst Evol Microbiol 50, 2231–2237.[CrossRef]
    [Google Scholar]
  8. Eaton, K. A., Dewhirst, F. E., Paster, B. J., Tzellas, N., Coleman, B. E., Paola, J. & Sherding, R. ( 1996; ). Prevalence and varieties of Helicobacter species in dogs from random sources and pet dogs: animal and public health implications. J Clin Microbiol 34, 3165–3170.
    [Google Scholar]
  9. Fox, J. G., Dewhirst, F. E., Tully, J. G., Paster, B. J., Yan, L., Taylor, N. S., Collins, M. J., Jr, Gorelick, P. L. & Ward, J. M. ( 1994; ). Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice. J Clin Microbiol 32, 1238–1245.
    [Google Scholar]
  10. Fox, J. G., Yan, L.-L., Dewhirst, F. E., Paster, B. J., Shames, B., Murphy, J. C., Hayward, A., Belcher, J. C. & Mendes, E. N. ( 1995; ). Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice. J Clin Microbiol 33, 445–454.
    [Google Scholar]
  11. Goodwin, C. S., Amstrong, J. A., Chilvers, T., Peters, M., Collins, M. D., Sly, L., McConnell, W. & Harper, W. E. S. ( 1989; ). Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov., respectively. Int J Syst Bacteriol 39, 397–405.[CrossRef]
    [Google Scholar]
  12. Hänninen, M.-L., Happonen, I., Saari, S. & Jalava, K. ( 1996; ). Culture and characteristics of Helicobacter bizzozeronii, a new canine gastric Helicobacter sp. Int J Syst Bacteriol 46, 160–166.[CrossRef]
    [Google Scholar]
  13. Harrington, C. S. & On, S. L. W. ( 1999; ). Extensive 16S rRNA gene sequence diversity in Campylobacter hyointestinalis strains: taxonomic and applied implications. Int J Syst Bacteriol 49, 1171–1175.[CrossRef]
    [Google Scholar]
  14. Hurtado, A. & Owen, R. J. ( 1997; ). A rapid identification scheme for Helicobacter pylori and other species of Helicobacter based on 23S rRNA gene polymorphism. Syst Appl Microbiol 20, 222–231.[CrossRef]
    [Google Scholar]
  15. Jalava, K., Kaartinen, M., Utriainen, M., Happonen, I. & Hänninen, M.-L. ( 1997; ). Helicobacter salomonis sp. nov., a canine gastric Helicobacter sp. related to Helicobacter felis and Helicobacter bizzozeronii. Int J Syst Bacteriol 47, 975–982.[CrossRef]
    [Google Scholar]
  16. Jalava, K., On, S. L. W., Vandamme, P. A., Happonen, I., Sukura, A. & Hänninen, M.-L. ( 1998; ). Isolation and identification of Helicobacter spp. from canine and feline gastric mucosa. Appl Environ Microbiol 64, 3998–4006.
    [Google Scholar]
  17. Jalava, K., Hielm, S., Hirvi, U. & Hänninen, M.-L. ( 1999; ). Evaluation of a molecular identification scheme based on 23S rRNA gene polymorphisms for differentiating canine and feline gastric Helicobacter spp. Lett Appl Microbiol 28, 269–274.[CrossRef]
    [Google Scholar]
  18. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  19. Kirkbride, C. A., Gates, C. E., Collins, J. E. & Ritchie, A. E. ( 1985; ). Ovine abortion associated with an anaerobic bacterium. J Am Vet Med Assoc 186, 789–791.
    [Google Scholar]
  20. Kirkbride, C. A., Gates, C. E. & Collins, J. E. ( 1986; ). Abortion in sheep caused by a nonclassified, anaerobic, flagellated bacterium. Am J Vet Res 47, 259–262.
    [Google Scholar]
  21. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  22. Lee, A., Phillips, M. W., O'Rourke, J. L. & 8 other authors ( 1992; ). Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int J Syst Bacteriol 42, 27–36.[CrossRef]
    [Google Scholar]
  23. Lockard, V. G. & Boler, R. K. ( 1970; ). Ultrastructure of a spiraled microorganism in the gastric mucosa of dogs. Am J Vet Res 31, 1453–1462.
    [Google Scholar]
  24. Melito, P. L., Munro, C., Chipman, P. R., Woodward, D. L., Booth, T. F. & Rogers, F. G. ( 2001; ). Helicobacter winghamensis sp. nov., a novel Helicobacter sp. isolated from patients with gastroenteritis. J Clin Microbiol 39, 2412–2417.[CrossRef]
    [Google Scholar]
  25. Mendes, E. N., Queiroz, D. M. M., Dewhirst, F. E., Paster, B. J., Moura, S. B. & Fox, J. G. ( 1996; ). Helicobacter trogontum sp. nov., isolated from the rat intestine. Int J Syst Bacteriol 46, 916–921.[CrossRef]
    [Google Scholar]
  26. On, S. L. W. ( 2001; ). Taxonomy of Campylobacter, Arcobacter, Helicobacter and related bacteria: current status, future prospects and immediate concerns. J Appl Microbiol 90, 1S–15S.[CrossRef]
    [Google Scholar]
  27. Paster, B. J. & Dewhirst, F. E. ( 1988; ). Phylogeny of campylobacters, wolinellas, Bacteroides gracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int J Syst Bacteriol 38, 56–62.[CrossRef]
    [Google Scholar]
  28. Patterson, M. M., Schrenzel, M. D., Feng, Y., Xu, S., Dewhirst, F. E., Paster, B. J., Thibodeau, S. A., Versalovic, J. & Fox, J. G. ( 2000; ). Helicobacter aurati sp. nov., a urease-positive Helicobacter species cultured from gastrointestinal tissues of Syrian hamsters. J Clin Microbiol 38, 3722–3728.
    [Google Scholar]
  29. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  30. Riley, L. K., Franklin, C. L., Hook, R. R., Jr & Besch-Williford, C. ( 1996; ). Identification of murine helicobacters by PCR and restriction enzyme analyses. J Clin Microbiol 34, 942–946.
    [Google Scholar]
  31. Robertson, B. R., O'Rourke, J. L., Vandamme, P., On, S. L. W. & Lee, A. ( 2001; ). Helicobacter ganmani sp. nov., a urease-negative anaerobe isolated from the intestines of laboratory mice. Int J Syst Evol Microbiol 51, 1881–1889.[CrossRef]
    [Google Scholar]
  32. Romero, S., Archer, J. R., Hamacher, M. E., Bologna, S. M. & Schell, R. F. ( 1988; ). Case report of an unclassified microaerophilic bacterium associated with gastroenteritis. J Clin Microbiol 26, 142–143.
    [Google Scholar]
  33. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  34. Saunders, K. E., Shen, Z., Dewhirst, F. E., Paster, B. J., Dangler, C. A. & Fox, J. G. ( 1999; ). Novel intestinal Helicobacter species from cotton-top tamarins (Saguinus oedipus) with chronic colitis. J Clin Microbiol 37, 146–151.
    [Google Scholar]
  35. Schauer, D. B., Ghori, N. & Falkow, S. ( 1993; ). Isolation and characterization of “Flexispira rappini” from laboratory mice. J Clin Microbiol 31, 2709–2714.
    [Google Scholar]
  36. Solnick, J. V. & Schauer, D. B. ( 2001; ). Emergence of diverse Helicobacter species in the pathogenesis of gastric and enterohepatic diseases. Clin Microbiol Rev 14, 59–97.[CrossRef]
    [Google Scholar]
  37. Sorlin, P., Vandamme, P., Nortier, J., Hoste, B., Rossi, C., Pavlof, S. & Struelens, M. J. ( 1999; ). Recurrent “Flexispira rappini” bacteremia in an adult patient undergoing hemodialysis: case report. J Clin Microbiol 37, 1319–1323.
    [Google Scholar]
  38. Steele, T. W. & McDermott, S. N. ( 1984; ). The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from feces. Pathology 16, 263–265.[CrossRef]
    [Google Scholar]
  39. Suerbaum, S., Kraft, C., Dewhirst, F. E. & Fox, J. G. ( 2002; ). Helicobacter nemestrinae ATCC 49396T is a strain of Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989 , and Helicobacter nemestrinae Bronsdon et al. 1991 is therefore a junior heterotypic synonym of Helicobacter pylori. Int J Syst Evol Microbiol 52, 437–439.
    [Google Scholar]
  40. Tee, W., Leder, K., Karroum, E. & Dyall-Smith, M. ( 1998; ).Flexispira rappini” bacteremia in a child with pneumonia. J Clin Microbiol 36, 1679–1682.
    [Google Scholar]
  41. Utriainen, M., Jalava, K., Sukura, A. & Hänninen, M.-L. ( 1997; ). Morphological diversity of cultured canine gastric Helicobacter spp. Comp Immunol Microbiol Infect Dis 20, 285–297.[CrossRef]
    [Google Scholar]
  42. Vandamme, P., Pot, B., Gillis, M., de Vos, P., Kersters, K. & Swings, J. ( 1996; ). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407–438.
    [Google Scholar]
  43. Vandamme, P., Harrington, C. S., Jalava, K. & On, S. L. W. ( 2000; ). Misidentifying helicobacters: the Helicobacter cinaedi example. J Clin Microbiol 38, 2261–2266.
    [Google Scholar]
  44. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  45. Weir, S., Cuccherini, B., Whitney, A. M. & 9 other authors ( 1999; ). Recurrent bacteremia caused by a “Flexispira”-like organism in a patient with X-linked (Bruton's) agammaglobulinemia. J Clin Microbiol 37, 2439–2445.
    [Google Scholar]
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