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Abstract

A fructophilic lactic acid bacterium, designated strain F214-1, was isolated from a flower of in South Africa. Based on phylogenetic analysis of 16S rRNA gene sequences, the strain formed a subcluster with and and, based on gene sequences, the strain formed a subcluster with . DNA–DNA hybridization studies showed that strain F214-1 was phylogenetically distinct from its closest relatives. Acid was produced from the fermentation of -glucose, -fructose and -mannitol only. -Fructose was the preferred sole carbon and energy source and was fermented more rapidly than -glucose. Growth of the strain on -glucose under anaerobic conditions was very weak but external electron acceptors such as oxygen and pyruvate enhanced growth on -glucose. Lactic acid and acetic acid were produced from -glucose in equimolar amounts. Ethanol was produced at very low levels, despite the strain’s obligately heterofermentative metabolism. Based on these data, strain F214-1 represents a novel species of fructophilic bacteria in the genus , for which the name sp. nov. is proposed. The type strain is F214-1 ( = JCM 16675  = DSM 23246).

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2011-04-01
2019-10-16
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vol. , part 4, pp. 898 - 902.

Phylogenetic relationship of sp. nov. F214-1 to related species based on 16S rRNA gene sequences using the maximum-parsimony method.

Phylogenetic relationship of sp. nov. F214-1 to related species based on 16S rRNA gene sequences using the maximum-likelihood method.

Growth rate of sp. nov. F214-1 on media containing various carbon sources.

Growth of F214-1 sp. nov. colonies on D-glucose under aerobic and anaerobic conditions after incubation for 2 days.

DNA-DNA relatedness between strain F214-1 and other species of the genus .

Supplementary Material [PDF](1.57MB).



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