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Abstract

A Gram-stain-positive endophytic bacterium, designated strain YC6957, was isolated from surface-sterilized roots of a halophyte ( Trin.) inhabiting coastal tidal flats of Namhae Island, located on the southern coast of Korea, and was subjected to a polyphasic taxonomic study. Cells were facultatively anaerobic, endospore-forming rods to coccoid rods, motile by a single flagellum. Strain YC6957 was catalase-positive, oxidase-negative and able to grow in the presence of 0–8 % (w/v) NaCl, with optimum growth at 4–5 % (w/v) NaCl. Growth occurred at 15–45 °C (optimal growth at 30–35 °C) and pH 6.0–8.5 (optimal growth at pH 7.0–8.0). The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were C (11.3 %), iso-C (19.2 %) and anteiso-C (36.4 %). The cell-wall peptidoglycan contained -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 41.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate belonged to the genus . Strain YC6957 exhibited high 16S rRNA gene sequence similarity to its closest neighbours, LMG 22866 (96.14 %), NCIMB 8773 (95.97 %) and LMG 17892 (95.91 %), and less than 95.84 % similarity to all other type strains in the genus . On the basis of the phylogenetic, physiological and biochemical data, it is suggested that strain YC6957 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YC6957 ( = KACC 13779  = DSM 22162).

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2011-07-01
2020-01-22
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Neighbour-joining and maximum-likelihood trees based on 16S rRNA gene sequences showing relationships between strain YC6957 and related species. [PDF](50 KB)

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Two-dimensional TLC of polar lipids of sp. nov. YC6957 (a) and DSM 15595 (b). First dimension, chloroform/methanol/water (65:25:4, by vol.); second dimension, chloroform/acetic acid/methanol/water (80:13:10:1.6, by vol.). DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; APL, unknown aminophospholipid; GL, unknown glycolipid; PL1–2, unknown phospholipids; L1–2, unknown lipids.

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