1887

Abstract

Two unknown, Gram-negative, catalase-negative and strictly anaerobic cocci were isolated from two independent human samples (strains AIP 49.01 and AIP 412.00). Comparative 16S rRNA gene sequencing demonstrated that these two organisms displayed 99·8 % sequence identity and that they are members of the sub-branch of the low-G+C Gram-positive bacteria. The most closely related 16S rDNA sequences were from sp. oral clone BU057 (99·8 %) and from isolates of and (94·5 and 93·8 %, respectively). Phylogenetic analysis based on 16S rDNA sequences showed that these two strains were most closely related to and belonged to the genus. Differences from previously described species in terms of size, biochemical tests (particularly the analysis of metabolic end products), gas production and DNA G+C content indicated that the two strains studied represent a novel species of anaerobic Gram-negative cocci. The name sp. nov. is proposed for these two isolates. It is also proposed that the uncultured organism previously deposited as sp. oral clone BU057 should be named ‘ Megasphaera micronuciformis’. The type strain of is AIP 412.00 (=CIP 107280 =CCUG 45952).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02378-0
2003-03-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/2/ijs530547.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02378-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Bergthorsson, U. & Ochman, H. ( 1998; ). Distribution of chromosome length variation in natural isolates of Escherichia coli. Mol Biol Evol 15, 6–16.[CrossRef]
    [Google Scholar]
  3. Bladen, H. A. & Mergenhagen, S. E. ( 1964; ). Ultrastructures of Veillonella and morphological correlation of an outer membrane with particles associated with endotoxic activity. J Bacteriol 88, 1482–1492.
    [Google Scholar]
  4. Carlier, J.-P. ( 1985; ). Gas chromatography of fermentation products: its application in diagnosis of anaerobic bacteria. Bull Inst Pasteur 83, 57–69.
    [Google Scholar]
  5. Carlier, J.-P., Marchandin, H., Jumas-Bilak, E., Lorin, V., Henry, C., Carrière, C. & Jean-Pierre, H. ( 2002; ). Anaeroglobus geminatus gen. nov., sp. nov., a novel member of the family Veillonellaceae. Int J Syst Evol Microbiol 52, 983–986.[CrossRef]
    [Google Scholar]
  6. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  7. Engelmann, U. & Weiss, N. ( 1985; ). Megasphaera cerevisiae sp. nov.: a new Gram-negative obligatory anaerobic coccus isolated from spoiled beer. Syst Appl Microbiol 6, 287–290.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1993; ). phylip (Phylogeny Inference Package) version 3.5c. Department of Genetics, University of Washington, Seattle, USA.
  9. Garrity, G. M. & Holt, J. G. ( 2001; ). Taxonomic outline of the Archaea and Bacteria. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 155–166. Edited by D. R. Boone & R. W. Castenholz. New York: Springer.
  10. Gupta, R. S. ( 1998; ). Protein phylogenies and signature sequences: a reappraisal of evolutionary relationships among Archaebacteria, Eubacteria, and Eukaryotes. Microbiol Mol Biol Rev 62, 1435–1491.
    [Google Scholar]
  11. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  12. Kalmokoff, M. L., Austin, J. W., Whitford, M. F. & Teather, R. M. ( 2000; ). Characterization of a major envelope protein from the rumen anaerobe Selenomonas ruminantium OB268. Can J Microbiol 46, 295–303.[CrossRef]
    [Google Scholar]
  13. Kamio, Y. & Takahashi, H. ( 1980; ). Outer membrane proteins and cell surface structure of Selenomonas ruminantium. J Bacteriol 141, 899–907.
    [Google Scholar]
  14. Liu, S.-L., Schryvers, A. B., Sanderson, K. E. & Johnston, R. N. ( 1999; ). Bacterial phylogenetic clusters revealed by genome structure. J Bacteriol 181, 6747–6755.
    [Google Scholar]
  15. Males, B. M., Berthold, P., Dougherty, P. A. & Listgarten, M. A. ( 1984; ). Helical flagellation in Centipeda periodontii, a Gram-negative, anaerobic bacillus from periodontitis lesions. J Gen Microbiol 130, 185–191.
    [Google Scholar]
  16. Marchandin, H., Jean-Pierre, H., Carrière, C., Canovas, F., Darbas, H. & Jumas-Bilak, E. ( 2001; ). Prosthetic joint infection due to Veillonella dispar. Eur J Clin Microbiol Infect Dis 20, 340–342.
    [Google Scholar]
  17. Morgenstern, B. ( 2002; ). A simple and space-efficient fragment-chaining algorithm for alignment of DNA and protein sequences. Appl Math Lett 15, 11–16.[CrossRef]
    [Google Scholar]
  18. Murray, R. G. E. & Schleifer, K. H. ( 1994; ). Taxonomic notes: a proposal for recording the properties of putative taxa of procaryotes. Int J Syst Bacteriol 44, 174–176.[CrossRef]
    [Google Scholar]
  19. Paster, B. J., Boches, S. K., Galvin, J. L., Ericson, R. E., Lau, C. N., Levanos, V. A., Sahasrabudhe, A. & Dewhirst, F. E. ( 2001; ). Bacterial diversity in human subgingival plaque. J Bacteriol 183, 3770–3783.[CrossRef]
    [Google Scholar]
  20. Reynolds, E. S. ( 1963; ). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17, 208–212.[CrossRef]
    [Google Scholar]
  21. Rogosa, M. ( 1971; ). Transfer of Veillonella Prévot and Acidaminococcus Rogosa from Neisseriaceae to Veillonellaceae fam. nov., and the inclusion of Megasphaera Rogosa in Veillonellaceae. Int J Syst Bacteriol 21, 231–233.[CrossRef]
    [Google Scholar]
  22. Rogosa, M. ( 1984; ). Anaerobic Gram-negative cocci. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 680–685. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  23. Rolph, H. J., Lennon, A., Riggio, M. P., Saunders, W. P., MacKenzie, D., Coldero, L. & Bagg, J. ( 2001; ). Molecular identification of microorganisms from endodontic infections. J Clin Microbiol 39, 3282–3289.[CrossRef]
    [Google Scholar]
  24. Skerman, V. B. D., McGowan, V. & Sneath, P. H. A. (editors) ( 1989; ). Approved Lists of Bacterial Names (amended edition). Washington, DC: American Society for Microbiology.
  25. Woods, C. R., Versalovic, J., Koeuth, T. & Lupski, J. R. ( 1993; ). Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. J Clin Microbiol 31, 1927–1931.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02378-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02378-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 547 - 553

Consensus phylogenetic tree based on 16S rRNA gene sequences, indicating the relationships between representative members of the sub-branch. Numbers given at nodes represent bootstrap percentages (1000 analyses). GenBank accession numbers are given in parentheses. Bar, 2 nucleotide substitutions per 100 nucleotides. [PDF](15 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error