Using a polyphasic approach, a taxonomic study was performed on seven strains of an unknown Gram-reaction-positive, non-spore-forming, obligately anaerobic coccus-shaped bacterium, isolated from a swine-manure storage pit. Comparative 16S rRNA gene sequencing confirmed that all seven isolates were highly related to each other and formed a hitherto unknown lineage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism was most closely related to Peptostreptococcus anaerobius CCUG 7835T and Peptostreptococcus stomatis CCUG 51858T with 16S rRNA gene sequence similarities of 95.5 and 93.0 %, respectively. The peptidoglycan type of the cell wall was determined to be A4α l-Lys–d-Asp and glucose, xylose and traces of mannose were detected as the cell–wall sugars. Based on biochemical, chemotaxonomic and phylogenetic evidence the unknown bacterium represents a new species of the genus Peptostreptococcus, for which the name Peptostreptococcus russellii sp. nov, is proposed. The type strain is RT-10BT ( = CCUG 58235T = NRRL B-59380T = DSM 23041T).
AttwoodG. T.,
KlieveA. V.,
OuwerkerkD.,
PatelB. K. C.1998; Ammonia-hyperproducing bacteria from New Zealand ruminants. Appl Environ Microbiol 64:1796–1804[PubMed]
CollinsM. D.,
LawsonP. A.,
WillemsA.,
CordobaJ. J.,
Fernandez-GarayzabalJ.,
GarciaP.,
CaiJ.,
HippeH.,
FarrowJ. A. E.1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [View Article][PubMed]
CottaM. A.,
WhiteheadT. R.,
ZeltwangerR. L.2003; Isolation, characterization and comparison of bacteria from swine faeces and manure storage pits. Environ Microbiol 5:737–745 [View Article][PubMed]
EzakiT.2009; Peptostreptococcaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriologyvol. 3 pp. 1008–1009 Edited by
De VosP.,
GarrityG. M.,
JonesD.,
KriegN. R.,
LudwigW.,
RaineyF. A.,
SchleiferK.-H.,
WhitmanW. B.
New York: Springer-Verlag;
EzakiT.,
KawamuraY.,
LiN.,
LiZ. Y.,
ZhaoL.,
ShuS.2001; Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus
. Int J Syst Evol Microbiol 51:1521–1528[PubMed]
HespellR. B.,
WolfR.,
BothastR. J.1987; Fermentation of xylans by Butyrivibrio fibrisolvens and other ruminal bacteria. Appl Environ Microbiol 53:2849–2853[PubMed]
JohnsonJ. L.1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology pp. 656–682 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: ASM;
KämpferP.,
KroppenstedtR. M.1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
MillerD. N.2001; Accumulation and consumption of odorous compounds in feedlot soils under aerobic, fermentative, and anaerobic respiratory conditions. J Anim Sci 79:2503–2512[PubMed]
MurdochD. A.,
ShahH. N.,
GharbiaS. E.,
RajendramD.2000; Proposal to restrict the genus Peptostreptococcus (Kluyver & van Niel 1936) to Peptostreptococcus anaerobius.
. Anaerobe 6:257–260 [View Article]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rDNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
TindallB. J.1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130[CrossRef]