1887

Abstract

Using a polyphasic approach, a taxonomic study was performed on seven strains of an unknown Gram-reaction-positive, non-spore-forming, obligately anaerobic coccus-shaped bacterium, isolated from a swine-manure storage pit. Comparative 16S rRNA gene sequencing confirmed that all seven isolates were highly related to each other and formed a hitherto unknown lineage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism was most closely related to CCUG 7835 and CCUG 51858 with 16S rRNA gene sequence similarities of 95.5 and 93.0 %, respectively. The peptidoglycan type of the cell wall was determined to be A4α -Lys–-Asp and glucose, xylose and traces of mannose were detected as the cell–wall sugars. Based on biochemical, chemotaxonomic and phylogenetic evidence the unknown bacterium represents a new species of the genus , for which the name sp. nov, is proposed. The type strain is RT-10B ( = CCUG 58235  = NRRL B-59380  = DSM 23041).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023762-0
2011-08-01
2019-10-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1875.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023762-0&mimeType=html&fmt=ahah

References

  1. Attwood G. T. , Klieve A. V. , Ouwerkerk D. , Patel B. K. C. . ( 1998; ). Ammonia-hyperproducing bacteria from New Zealand ruminants. . Appl Environ Microbiol 64:, 1796–1804.[PubMed]
    [Google Scholar]
  2. Bryant M. P. . ( 1972; ). Commentary on the Hungate technique for culture of anaerobic bacteria. . Am J Clin Nutr 25:, 1324–1328.[PubMed]
    [Google Scholar]
  3. Collins M. D. , Lawson P. A. , Willems A. , Cordoba J. J. , Fernandez-Garayzabal J. , Garcia P. , Cai J. , Hippe H. , Farrow J. A. E. . ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. . Int J Syst Bacteriol 44:, 812–826. [CrossRef].[PubMed]
    [Google Scholar]
  4. Cotta M. A. , Whitehead T. R. , Zeltwanger R. L. . ( 2003; ). Isolation, characterization and comparison of bacteria from swine faeces and manure storage pits. . Environ Microbiol 5:, 737–745. [CrossRef].[PubMed]
    [Google Scholar]
  5. Downes J. , Wade W. G. . ( 2006; ). Peptostreptococcus stomatis sp. nov., isolated from the human oral cavity. . Int J Syst Evol Microbiol 56:, 751–754. [CrossRef].[PubMed]
    [Google Scholar]
  6. Ezaki T. . ( 2009; ). Peptostreptococcaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriology, vol. 3, pp. 1008–1009. Edited by De Vos P. , Garrity G. M. , Jones D. , Krieg N. R. , Ludwig W. , Rainey F. A. , Schleifer K.-H. , Whitman W. B. . . New York:: Springer-Verlag;.
    [Google Scholar]
  7. Ezaki T. , Kawamura Y. , Li N. , Li Z. Y. , Zhao L. , Shu S. . ( 2001; ). Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus . . Int J Syst Evol Microbiol 51:, 1521–1528.[PubMed]
    [Google Scholar]
  8. Hespell R. B. , Wolf R. , Bothast R. J. . ( 1987; ). Fermentation of xylans by Butyrivibrio fibrisolvens and other ruminal bacteria. . Appl Environ Microbiol 53:, 2849–2853.[PubMed]
    [Google Scholar]
  9. Hungate R. E. . ( 1969; ). A roll tube method for cultivation of strict anaerobes. . Methods Microbiol 3:, 117–132. [CrossRef]
    [Google Scholar]
  10. Johnson J. L. . ( 1994; ). Similarity analysis of DNAs. . In Methods for General and Molecular Bacteriology, pp. 656–682. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: ASM;.
    [Google Scholar]
  11. Kämpfer P. , Kroppenstedt R. M. . ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  12. Lipman D. J. , Pearson W. R. . ( 1985; ). Rapid and sensitive protein similarity searches. . Science 227:, 1435–1441. [CrossRef].[PubMed]
    [Google Scholar]
  13. MacKenzie S. L. . ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. . J Assoc Off Anal Chem 70:, 151–160.[PubMed]
    [Google Scholar]
  14. Miller D. N. . ( 2001; ). Accumulation and consumption of odorous compounds in feedlot soils under aerobic, fermentative, and anaerobic respiratory conditions. . J Anim Sci 79:, 2503–2512.[PubMed]
    [Google Scholar]
  15. Murdoch D. A. , Shah H. N. , Gharbia S. E. , Rajendram D. . ( 2000; ). Proposal to restrict the genus Peptostreptococcus (Kluyver & van Niel 1936) to Peptostreptococcus anaerobius. . Anaerobe 6:, 257–260. [CrossRef]
    [Google Scholar]
  16. Nicholas K. B. , Nicholas H. B. Jr , Deerfield D. W. II . ( 1997; ). GeneDoc: analysis and visualization of genetic variation. . EMBnet News 4 (2), 1–4. http://www.embnet.org/download/embnetnews/embnet_news_4_2.pdf
    [Google Scholar]
  17. Page R. D. . ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. . Comput Appl Biosci 12:, 357–358.[PubMed]
    [Google Scholar]
  18. Rasmussen, S. W. (2002). seqtools, a software package for analysis of nucleotide and protein sequences. http://www.seqtools.dk
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Schleifer K. H. . ( 1985; ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  21. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  22. Stackebrandt E. , Ebers J. . ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33:, 152–155.
    [Google Scholar]
  23. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rDNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  24. Tindall B. J. . ( 1990a; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130.[CrossRef]
    [Google Scholar]
  25. Tindall B. J. . ( 1990b; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  26. Whitehead T. R. , Cotta M. A. . ( 2004; ). Isolation and identification of hyper-ammonia producing bacteria from swine manure storage pits. . Curr Microbiol 48:, 20–26. [CrossRef].[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023762-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023762-0
Loading

Data & Media loading...

Supplements

vol. , part 8, pp. 1875 - 1879

Polar lipid analysis of RT-10B .

16 rRNA gene sequence alignment of variable region 1 of , and . [PDF]



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error