1887

Abstract

A novel, Gram-stain-positive bacterial strain, Mer 29717, was isolated from the branchia (gills) of a Japanese codling, , collected from bottom waters of Suruga Bay in Shizuoka, Japan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain represents a distinct lineage within the family and was related most closely to members of the genera and . It shared highest 16S rRNA gene sequence similarity (95.1 %) with YIM 45900. Strain Mer 29717 contained MK-8(H) and MK-8(H) as menaquinones, and iso-C, C, C -9, C, C -9 and C -10 were the major cellular fatty acids. The cell-wall peptidoglycan of strain Mer 29717 was composed of -Lys, -Ser, -Ser, Gly, -Glu and -Ala. Polar lipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and one unidentified phospholipid. Mycolic acids were not detected. The G+C content of the DNA of strain Mer 29717 was 68 mol%. On the basis of differential chemotaxonomic, physiological and biochemical data, strain Mer 29717 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is Mer 29717 ( = NBRC 106121  = DSM 22951).

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2011-05-01
2020-01-17
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Polar lipid profile of strain Mer 29717 by two-dimensional TLC with detection by Dittmer–Lester reagent. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol; L1, unidentified polar lipid.

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Maximum-likelihood tree showing the phylogenetic positions of strain Mer 29717 and related organisms, inferred from 16S rRNA gene sequence analyses. [PDF](45 KB)

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