1887

Abstract

A novel strictly anaerobic, cellobiose-degrading bacterium, strain Cello, was isolated from a human faecal sample by combining enrichments in liquid and soft-agar basal media. A noteworthy characteristic was its inability to grow on normal agar plates and in roll tubes. The cells were coccus shaped and non-motile, with an extracellular slime layer. Growth of strain Cello occurred between 20 and 40 °C, with optimal growth at 37 °C. The pH range for growth was 5–7·5 with an optimum at 6·5. In pure culture, strain Cello could only grow on a variety of sugars. Glucose was converted to acetate, ethanol and H. The doubling time on glucose was 0·5 h. In a syntrophic co-culture with strain JF-1, strain Cello converted glucose to acetate and H. The G+C content was 59·2 mol%. 16S rDNA analysis revealed that the closest relatives of strain Cello were two uncultured bacteria from anaerobic digesters, both with 94 % 16S rDNA sequence similarity. The closest cultured representatives belong to genera of the bacterial division ‘’. The name gen. nov., sp. nov. is proposed for strain Cello (=DSM 14823 =ATCC BAA-548).

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2003-01-01
2019-10-23
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