@article{mbs:/content/journal/ijsem/10.1099/ijs.0.023614-0, author = "Im, Wan-Taek and Yang, Jung-Eun and Kim, Se-Young and Yi, Tae-Hoo", title = "Chryseobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a Rhus vernicifera-cultivated field", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2011", volume = "61", number = "6", pages = "1430-1435", doi = "https://doi.org/10.1099/ijs.0.023614-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.023614-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain, designated THG 15T, was isolated from soil of a field cultivated with Rhus vernicifera in Okcheon province, South Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain THG 15T grew optimally at 25–30 °C and at pH 7 in the absence of NaCl on nutrient agar. Strain THG 15T displayed β-glucosidase (aesculinase) activity that was responsible for its ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) into compound K via Rd and F2. On the basis of 16S rRNA gene sequence similarities, strain THG 15T was shown to belong to the family Flavobacteriaceae and was most closely related to Chryseobacterium soldanellicola PSD1-4T (97.7 % sequence similarity), Chryseobacterium soli JS6-6T (97.5 %) and Chryseobacterium indoltheticum LMG 4025T (97.3 %). The G+C content of the genomic DNA was 35.7 mol%. The major menaquinone was MK-6 and the major fatty acids were iso-C15 : 0 (50.3 %), iso-C17 : 0 3-OH (21.9 %), summed feature 4 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH; 9.5 %) and iso-C17 : 1ω9c (9.3 %). DNA sequence analysis and chemotaxonomic data supported the affiliation of strain THG 15T to the genus Chryseobacterium. DNA–DNA relatedness values between strain THG 15T and its closest phylogenetic neighbours were <15 %. Strain THG 15T could be differentiated genotypically and phenotypically from recognized species of the genus Chryseobacterium. The isolate therefore represents a novel species, for which the name Chryseobacterium ginsenosidimutans sp. nov. is proposed. The type strain is THG 15T ( = KACC 14527T  = JCM 16719T).", }