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Volume 61, Issue 10

Usefulness of a real-time PCR platform for G+C content and DNA–DNA hybridization estimations in vibrios Free

Abstract

The aim of this study was to evaluate the utility of a real-time PCR platform to estimate the DNA G+C content (mol%) and DNA–DNA hybridization (DDH) values in the genus . In total, nine vibrio strains were used to determine the relationship between genomic DNA G+C content and (°C). The and HPLC datasets fit a linear regression curve with a significant correlation coefficient, corroborating that this methodology has a high correlation with the standard methodology based on HPLC (R = 0.94). Analysis of 31 pairs of vibrios provided a wide range of Δ values, varying between 0.72 and 12.5 °C. Pairs corresponding to strains of the same species or strains from sister species showed the lowest Δ values. For instance, the Δ of the sister species LMG 4044 and LMG 11216 was 5.2 °C, whereas the Δ of LMG 20984 and LMG 20536 was 8.75 °C. The mean Δ values corresponding to pairs of strains with DDH values lower than 60 % or higher than 80 % were, respectively, 8.29 and 2.21 °C (significant difference, <0.01). The high correlation between DDH values obtained in previous studies and the Δ values (R = 0.7344) indicates that the fluorimetric methodology is a reliable alternative for the estimation of both DNA G+C content and Δ in vibrios. We suggest that strains of the same species will have less than 4 °C Δ . The use of a real-time PCR platform represents a valuable alternative for the development of the taxonomy of vibrios.

  • Published Online:
Funding
This study was supported by the:
  • CNPq
  • CAPES
  • FAPERJ
  • IFS
© 2011 IUMS
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2011-10-01
2024-04-19
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Usefulness of a real-time PCR platform for G+C content and DNA–DNA hybridization estimations in vibrios
Int J Syst Evol Microbiol 61, 2379 (2011); https://doi.org/10.1099/ijs.0.023606-0
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